同时测定唇彩中苏丹红Ⅰ和Ⅱ的高效液相色谱法的建立  

A Method of Simultaneous Determination for Sudan RedⅠandⅡin Lip Gloss by High-performance Liquid Chromatography

作　　者：徐明敏[1] 杨毅华[1] 陈波[1] 

机构地区：[1]宁波市北仑区疾病预防控制中心,浙江宁波315800

出　　处：《浙江预防医学》2013年第2期12-15,共4页Zhejiang Journal of Preventive Medicine

摘　　要：目的建立准确、灵敏的高效液相色谱法同时测定唇彩中苏丹红Ⅰ和苏丹红Ⅱ的分析方法。方法样品经乙腈-甲醇-氯仿(体积比为2∶1∶1)混合溶剂提取后,在C18色谱柱上,以0.1%乙酸溶液和含0.1%乙酸的乙腈溶液作为流动相,采用梯度洗脱分离,在476 nm波长进行检测。结果苏丹红Ⅰ和苏丹红Ⅱ的加标回收率在86.2%～106.2%范围,其相对标准偏差均小于5.0%,在0.2～20.0 mg/L范围呈现良好的线性关系,其回归系数大于0.999,检出限(LOD)分别为0.02和0.03 mg/kg,最低定量检出限(LOQ)分别为0.07和0.10 mg/kg。结论本方法简便、灵敏、重现性好,能满足唇彩中苏丹红Ⅰ和Ⅱ残留的监测要求。Objective To develop a method for the simultaneous determination of Sudan Red I and Sudan Red H in Lip Gloss by high - performance liquid chromatography. Methods After extracted by a mixed solvent of acetonitrile - methanol - chloroform （2：1 ： 1, V/V/V）, the sample was separated on a C18 column by a mobile phase consisting of 0. 1% acetic acid （A） and acetonitrile with 0. 1% acetic acid （B） in gradient elation. Detection was carried out by a visible detector at 476 nm. Results Calibration carves of Sudan Red I and Sudan Red R were linear within the range of 0. 2 mg/L ～ 20. 0 mg/L with correlation coefficients of more than 0. 999. The limits of detection （LOD） were 0. 02 mg/kg and 0. 03 mg/kg, the limits of quantification （LOQ） were 0. 07 mg/kg and 0. 10 mg/kg for Sudan ROd I and Sudan Red II respectively. The extraction recoveries were between 86.2% ～ 106. 2%, and the RSDs were less than 5.0%. Conclusion This method is simple, sensitive, accurate and good specificity for monitoring the residues of Sudan Red Ⅰ and Ⅱ in Lip Gloss.

关 键 词：液相色谱法 苏丹红Ⅰ 苏丹红Ⅱ 唇彩 残留测定 

分 类 号：R730.43[医药卫生—肿瘤]