三种查尔酮类化合物与人血清白蛋白相互作用及其构效关系研究  

Study on the Interaction and Structure-activity Relationship between Three Chalcones and Human Serum Albumin

作　　者：张爱平[1] 郝娟[1] 黄茜[1] 高晓亚[1] 文雯[1] 

机构地区：[1]山西医科大学药学院,山西太原030001

出　　处：《分析科学学报》2013年第1期73-77,共5页Journal of Analytical Science

基　　金：山西省自然科学基金(No.2010011048-1);山西医科大学科技创新基金(No.01200806)

摘　　要：在模拟人体生理条件下,采用紫外光谱法、荧光光谱法和同步荧光光谱法研究查尔酮、4′-甲氧基查尔酮和4′-氯查尔酮与人血清白蛋白(HSA)的相互作用及其构效关系。实验表明:三种查尔酮类化合物对HSA的荧光猝灭机制主要为静态猝灭,与HSA均形成1∶1复合物,结合常数K分别为2.50×104、0.697×104和0.277×104 L.moL-1,结合距离r分别为3.78、3.93和4.25nm,其作用力均以氢键和范德华力为主。查尔酮类化合物中取代基的不同对其与HSA的结合产生影响,其作用力大小依次为查尔酮>4′-甲氧基查尔酮>4′-氯查尔酮。The interaction and structure-activity relationship of human serum albumin with chalcone,4＇- methoxychalcone and 4＇-chlorochalcone were investigated by ultraviolet spectroscopy, fluorescence spectroscopy and synchronous fluorescence spectroscopy under the simulative human physiological conditions. The binding constants, the number of binding sites and the thermodynamic parameters were calculated and the effects of the three compounds on the conformation of HSA were studied. The results showed that the intrinsic fluorescence of HSA was significantly quenched by chalcone, 4＇- methoxychalcone and 4＇-chlorochalcone, respectively. The mechanism of fluorescence quenching was mainly static quenching with non-radiation energy transfer. The 1＂ 1 compound was formed between each chalcone and HSA. The binding parameters of chalcone,4＇-methoxychalcone and 4＇ chloroehalcone with HSA were as follows=the binding constants K were 2.50）〈 104 ,6. 97X 103 and 2.77X 10aL.moL 1,the binding distances r were 3.78,3.93 and 4.25 nm, respectively. The driving forces were mainly hydrogen bond and Vander Waals according to the thermodynamic parameters. The synchronous fluorescence spectra indicated that the binding sites of chalcone,4＇-methoxychalcone and 4＇-chlorochalcone with HSA were closer to tryptophan residues. The interaction of the three compounds with HSA could result in the decrease of tryptophan residues hydrophobicity and the change of HSA conformation. The results demonstrated that the different substituent in chalcones played an important role in the interaction between chalcones and HSA,and the order of the interaction strength between chalcones and HSA were chalcone〉4＇-methoxychalcone 〉4＇-chlorochalcone.

关 键 词：查尔酮 4’甲氧基查尔酮 4’-氯查尔酮 人血清白蛋白 紫外光谱法 荧光光谱 法 同步荧光光谱法 

分 类 号：O657.39[理学—分析化学]