DC-CIK共培养在AL化疗后免疫治疗中的应用及其对NKG2D表达的影响  

Effect of dentritic cells-cytokine-induced killer cells coculture therapy on NKG2D after chemotherapy of acute leukemia

作　　者：杨皎娃[1] 邓琦[1] 李玉明[1] 

机构地区：[1]天津市第一中心医院,天津300192

出　　处：《山东医药》2013年第2期1-3,共3页Shandong Medical Journal

基　　金：天津市卫生局科技基金资助项目(2011ky07)

摘　　要：目的观察树突状细胞(DC)—细胞因子诱导的杀伤细胞(CIK)共培养用于急性白血病(AL)化疗后免疫治疗的疗效,及其对自然杀伤(NK)细胞表面活化受体NKG2D表达的影响。方法 31例AL患者经诱导治疗骨髓达完全缓解后,给予DC-CIK共培养治疗。治疗前后检测外周血中T细胞亚群表达、血清IL-2、IL-12、TNF-α、IFN-γ水平及外周血中NKG2D的表达。结果所有患者治疗结束后骨髓均处于完全缓解状态;DC-CIK共培养治疗后,CD3+、CD3+CD8+、CD3+CD5+6/CD1+6高于治疗前(P均<0.05);血清IL-2、IL-12、TNF-α、IFN-γ水平高于治疗前(P均<0.05);外周血单个核细胞中NKG2D阳性表达率为25.41%±8.47%,高于治疗前的8.07%±2.02%(P<0.05)。结论 DC-CIK共培养用于AL化疗后的免疫治疗效果较好,能更有效地清除微小残留病灶,提高外周血中NKG2D的表达,纠正AL细胞对自体NK细胞的免疫编辑作用。Objective To investigate the effect of dentritic cells-cytokine induced killer ceils （DC-CIK） cocultrue ther- apy on nature killer cell group 2, member D （NKG2D）. Methods DC-CIK cocultrue therapy was adopted in 31 acute leu- kemia （AL） patients when they were in complete remission after inductive treatment. The levels oft cell subsets（including CD3＋ ,CD3＋CD8＋ , CD3＋CD56＋/CD16＋）, IL-2,IL-12,TNF,IFN-γand the expression of NKG2D were observed before and after the treatment. Results All patients were in complete remmission after threatment. The expression of CD3＋, CD3＋CD8＋ , CD3＋ CD56＋/CD16＋ were higher than that of treatment before. The positive rate of NKG2D in peripheral blood was 25.41% ± 8.47% , which was higher than that of treatment before （ that was 8.07% ± 2.02% ）. Conclusion DC-CIK eocultrue treatment is effective in immunotherapy after the chemotherapy of AL, by which the expression of NKG2D enhanced, immu- nity edition of NK cells corrected.

关 键 词：急性白血病 树突状细胞 细胞因子诱导的杀伤细胞 自然杀伤细胞 

分 类 号：R733.7[医药卫生—肿瘤]