铁苋菜多糖体外抗氧化研究  

Antioxidant Activities in vitro of Polysaccharides from Acalypha australis

作　　者：魏秀娟[1] 向发椿[1] 崔明筠[1] 徐健蓉[1] 

机构地区：[1]西南科技大学生命科学与工程学院,四川绵阳621010

出　　处：《中国实验方剂学杂志》2013年第3期197-200,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：西南科技大学博士基金项目(12zx7108)

摘　　要：目的:研究不同醇沉浓度下铁苋菜多糖的体外抗氧化活性。方法:用质量浓度为30%,45%,60%,75%,80%的无水乙醇分级沉淀的方法得到不同组分的铁苋菜多糖,分别采用高铁还原法、对二苯代苦味酰基(DPPH.)体系、光照核黄素法、硫代巴比妥酸法比较它们的总还原力(T-AOC)、对二苯代苦味酰基自由基(DPPH.)、超氧阴离子自由基(O2-.)、羟基自由基(.OH)的清除作用及对卵黄脂蛋白脂质过氧化物(LPO)的抑制效果。结果:醇沉浓度为75%时对DPPH.的清除率达到了73.85%,其余各组分多糖都有良好的抗氧化效果,其中对O2-.和.OH的清除作用最为明显,清除率分别达到了92.47%和95.89%,几乎与抗坏血酸的清除作用相当,75%醇沉条件下的多糖对LPO也有较强的抑制作用。结论:铁苋菜各组分多糖都有较强的抗氧化作用。Objective： To assess the antioxidant activities of polysaccharide from Acalypha australis L. by different ethanol concentration in vitro. Method： The different part of po!ysaccharide were precipitated by 30% , 45%, 60%, 75%, 80% ethanol. The scavenging rate of the precipitated polysaccharides on （DPPH）free radical, superoxide anion radical （O2- ）, hydroxyl radicals （-OH） was investigated by ferric reducing method, DPPH- radical assay, 02 radical assay and thiobarbituric acid method respectively. The total antioxidantive capacity （T-AOC） and the inhibition on lipid peroxide （LPO） of the precipitated polysaccharides were also been determined. Result： The scavenging activities on the DPPH ~ reached 73.85%. The results showed that T-AOC was enhanced with the increase of polysaccharide concentration. The antioxidantive especially the scavenging activities on the 02 and OH reached 92.47% , 95.89% respectively at 5 g L-1. Furthermore the scavenging effects were as much as that of vitamin C （VC）. The inhibition of LPO with the polysaccharide precipitated by 75% ethanol was significantly higher than that of any by other ethanol concentrations. Conclusion： the precipitated polysaccharides from A. australis showed strong antioxidant activities.

关 键 词：铁苋菜多糖 分级沉淀 抗氧化性 自由基 

分 类 号：R285.5[医药卫生—中药学]