吡格列酮对STZ糖尿病大鼠肾组织氧化应激的影响  

Effect of different dosages of piogtitazone on the oxidative stress in the kidney of STZ-induced diabetic rats

作　　者：王月芬[1] 叶山东[1] 邢燕[1] 

机构地区：[1]安徽医科大学附属省立医院安徽省立医院内分泌科,安徽合肥230001

出　　处：《中国药理学通报》2013年第1期85-88,共4页Chinese Pharmacological Bulletin

基　　金：安徽省自然科学基金资助项目(No 070413255X);安徽省人才开发基金资助(No 2008Z048)

摘　　要：目的观察不同剂量盐酸吡格列酮对STZ诱导的糖尿病大鼠肾组织氧化应激的影响。方法 STZ腹腔注射建立糖尿病大鼠模型。成模糖尿病大鼠随机分为模型组和不同剂量吡格列酮组,并设正常对照组,干预8周后检测肾皮质MDA含量,SOD活性,肾组织NADPH氧化酶亚单位p22phoxmRNA和p47phox mRNA表达。结果各吡格列酮组较模型组MDA含量,p22phox mRNA和p47phox mRNA表达明显降低,SOD活性明显升高(P<0.05)。结论吡格列酮可抑制STZ糖尿病大鼠肾脏NADPH氧化酶表达,降低肾脏氧化应激水平,并具有一定的剂量依赖性,该作用可能与其肾脏保护部分有关。Aim To observe the effect of different dos- ages of pioglitazone on the oxidative stress in the kid- ney of STZ-induced diabetic rats. Methods Diabetic models were established by a single injection of strepto- zotocin. All the diabetic rats were randomly divided in- to following groups： diabetic rats without treatment, different dosages of pioglitazone treated diabetic rats, healthy rats served as a normal control group. At the 8 th week, the renal tissue was acquired for measuring malonaldehyde （ MDA ） level, expression of p22phox mRNA and p47phox mRNA, activities of superoxidedismutase （SOD）Result Compared with model group, MDA level, expression of p22phox mRNA and p47phox mRNA had decreased and SOD activity had increased significantly in pioglitazone group （P 〈 0.05）. Conclusion Pioglitazone can inhibit NADPH oxidase expression, lower the oxidative stress in the kidney in a dose-independent manner to some extent, which may play a reno-protective role in STZ-induced diabetic rats.

关 键 词：糖尿病肾病 氧化应激 NADPH氧化酶 MDA SOD 吡格列酮 

分 类 号：R-332[医药卫生] R322.61