白介素-15对过氧化氢诱导大鼠Ⅱ型肺泡上皮细胞凋亡的影响  

Influence of interleukin-15 on the rat type Ⅱ alveolar epithelial cells apoptosis induced by hydrogen peroxide

作　　者：陈子盛[1] 傅应云[1] 刘盛国[1] 何正强[1] 

机构地区：[1]暨南大学第二临床医学院深圳市人民医院呼吸科,广东深圳518020

出　　处：《中国药理学通报》2013年第1期132-135,共4页Chinese Pharmacological Bulletin

摘　　要：目的观察白介素-15(IL-15)对H2O2诱导的大鼠Ⅱ型肺泡上皮细胞凋亡的影响及探讨其机制。方法体外培养大鼠Ⅱ型肺泡上皮细胞(RLE-6TN),分为:(A)正常对照组;(B)IL-15预处理组;(C)H2O2损伤组;(D)IL-15预处理后H2O2损伤组。采用DAPI荧光染色及Annexin V-FITC/PI检测各组细胞凋亡,JC-1染色观察各组线粒体膜电位;RT-PCR检测各组细胞Bcl-2、Bax、Caspase-3 mRNA表达。结果与正常对照组比较,H2O2损伤能明显诱导RLE-6TN凋亡,IL-15预处理可明显降低H2O2诱导的细胞凋亡率,使细胞线粒体膜电位下降,Bcl-2 mRNA表达增加,Bax、Caspase-3mRNA表达降低(P<0.05)。结论 IL-15能抑制H2O2诱导的大鼠Ⅱ型肺泡上皮细胞凋亡,其作用机制可能为稳定细胞线粒体膜电位,增强抗凋亡基因Bcl-2,同时,抑制促凋亡基因Bax表达,下调Caspase-3表达。Aim To study the effect of interleukin-15 （IL-15） on the rat type Ⅱalveolar epithelial cells （RLE-6TN） apoptosis induced by hydrogen peroxide （H202 ）. Methods The experimental RLE-6TN were cultured in vivo, which were divided into four groups： （A） normal control group; （B） pre-treated group with IL-15; （C） injured group with H202; （D）the group pre-treated with IL-15 and injured successively by H202. Cell apoptosis was detected by DAPI staining and Annexin V-FITC/PI; the change of mitochondrial membrane potential was observed by JC-1 staining; the expression of Bcl-2, Bax and Caspase-3 mRNA of RLE-6TN were detected by RT-PCR. Results Com-pared with the control group, H202 induced cells apop- tosis obviously （P 〈 0.05）. But IL-15 significantly re- duced the rate of H202-induced cell apoptosis, mito- chondrial membrane potential and expression of Bax and Caspase-3 mRNA. While it significantly increased the expression of Bcl-2 mRNA. Conclusion IL-15 inhibits the H2 02 -induced apoptosis of type Ⅱ alveolar epithelial cells. It may protect the mitochondrial mem- brane potential, and promot the gene expression of Bcl- 2, inhibit the Bax and Caspase-3 expression.

关 键 词：白介素-15 Ⅱ型肺泡上皮细胞 氧化损伤 细胞凋 亡 抗凋亡 机制 

分 类 号：R-332[医药卫生] R322.35