DNA氧化损伤在翼状胬肉发病中的作用  

Effects of oxidative damage of DNA on pathogenesis of pterygium

作　　者：赵博[1] 吴江[1] 景红[1] 王永毅[1] 

机构地区：[1]河南大学淮河医院眼科,开封475000

出　　处：《中华实验眼科杂志》2013年第2期160-163,共4页Chinese Journal Of Experimental Ophthalmology

摘　　要：背景翼状胬肉是人眼表常见疾病，其确切的病因尚未明确，有关翼状胬肉发病机制的研究仍是临床关注的重点。目的检测DNA氧化损伤标志物8-羟基脱氧鸟苷（8-OHdG）和凋亡相关基因bcl-2在翼状胬肉和正常结膜组织中的表达，探讨DNA氧化损伤在翼状胬肉发病机制中的可能作用。方法在翼状胬肉切除手术中收集30例翼状胬肉组织标本，其中原发性翼状胬肉标本24例，复发性翼状胬肉标本6例。另收集行视网膜脱离手术、斜视手术和上睑下垂手术的正常球结膜标本20例。免疫组织化学法检测翼状胬肉标本中8-OHdG和bcl-2的表达，并与正常球结膜组织的标本进行对照。用卡方检验法比较各组标本中8-OHdG和bcl-2阳性表达的差异，用Kappa一致性检验评价翼状胬肉组织中8-OHdG和bcl-2阳性表达的一致性。结果原发性和复发性翼状胬肉组织中8-OHdG的阳性表达率分别为62．5％和83．3％，而正常结膜组织中8．OHdG无表达；原发性和复发性翼状胬肉组织中8-OHdG阳性表达率的比较差异无统计学意义（x2=0．938，P〉0．05）。原发性和复发性翼状胬肉组织中bcl-2的阳性表达率分别为90．O％和87．5％，而正常结膜组织中bcl-2无表达；原发性和复发性翼状胬肉组织中bcl-2的阳性表达率差异无统计学意义（，=0．833，P〉0．05）。27例bcl-2阳性的翼状胬肉标本中有20例8-OHdG呈阳性表达（20／27），3例bcl-2阴性的翼状胬肉标本8-OHdG均呈阴性表达，二者的表达结果差异无统计学意义（P〉0．05）。翼状胬肉标本中8-OHdG与bcl-2的表达一致性一般（Kappa=0．464）。结论翼状胬肉组织中8-OHdG呈阳性表达，提示DNA氧化损伤在翼状胬肉的发病机制中有一定作用，紫外线所致的DNA氧化损伤可能作为上游的因素，导致凋亡相关基因bcl-2的增高，从而抑制了正常的细胞凋亡，造成组织细胞的过度增生，导致了�Background Pterygium is a relatively common eye disease,but its aetiology and pathogenesis remain uncertain. At present,the study on pterygia focuses on understanding its underlying mechanism. Objective This study was to detect the expression of 8-hydroxy-2＂-deoxyguaine （8-OHdG） , a marker of oxidative damage of DNA,and bcl-2, a gene related with apoptosis, on the pterygium tissue. Methods Thirty pterygium tissue specimens were obtained during the surgery with the primary pterygium 24 cases and recurrent pterygium 6 cases. In addition ,20 normal eonjunctival specimens from retinal detachment surgery and strabismus surgery were collected. The expressions of 8-OHdG and bcl-2 in pterygium tissue were detected using immunochemistry and compared with the normal conjunctival tissue. The difference in the expressions of 8-OHdG and bel-2 among different specimens was compared by x2test,and the relationship between 8-OHdG expression and bcl-2 expression was evaluated by Kappa test. Results The positive expressing rate of 8-OHdG in the pterygium tissue was 62.5% and 83.3% in the primary and recrudescence pterygium tissue, respectively, but the expression of 8-OHdG was absent in the normal eonjunetiva tissue. No significant difference was found in the positive expressing rate of 8-OHdG between primary and recrudescence pterygium tissue（x2 = 0. 938, P〉0.05 ）. The bcl-2 expressing rate was 90.0% and 87.5% in the primary and recrudescence pterygium tissue,respectively. However,that in the normal conjunctival tissue was absent. No significant difference was seen in the bcl-2 expression rate between primary and recrudescence pterygium tissue （X2= 0. 833, P〉 0. 05 ）. Of the 27 pterygium tissue with bcl-2 positive expression, 8-OHdG showed the positive expression in 20 specimens, and 3 specimens with the bcl-2 negative response were absent reactive to 8-OHdG, showing insignificant difference between them （P〉0.05）. The relationship between 8-OHdG expression and bcl-2 expression was concord in a certein exten

关 键 词：翼状胬肉 8-羟基脱氧鸟苷 氧化损伤 凋亡相关基因 BCL-2 

分 类 号：R779.6[医药卫生—眼科]