Cloning and bioinformatical analysis of the N-terminus of the sonic hedgehog gene  

作　　者：Yi Zhang Shu Zhao Weiren Dong Suifen He Haihong Wang Lihua Zhang Yinjuan Tang Jiasong Guo Suiqun Guo 

机构地区：[1]School of Basic Medical Sciences,Southern Medical University [2]Department of Gynecology and Obstetrics,the Third Affiliated Hospital of Southern Medical University [3]Department of Laboratory,Shunde Guizhou Hospital [4]School of Medical Sciences,Foshan University [5]Department of Histology & Embryology,Xiangnan College

出　　处：《Neural Regeneration Research》2013年第3期258-263,共6页中国神经再生研究（英文版）

基　　金：sponsored by the Guangdong Provincial Natural Science Foundation,No.S2012010009592;the Science and Technology Talent Foundation of Guangdong Provincial Natural Science Foundation,No.30900725;the Joint Research Program by Southern Medical University-Shunde Guizhou Hospital,No.09000608;the Science Foshan Municipal Key Project in Medical Sciences,No.201008063;and the Shunde Medical Research Program,No.2011050

摘　　要：The sonic hedgehog protein not only plays a key role in early embryonic development, but also has essential effects on the adult nervous system, including neural stem cell proliferation, differentiation migration and neuronal axon guidance. The N-terminal fragment of sonic hedgehog is the key functional element in this process. Therefore, this study aimed to clone and analyze the N-terminal fragment of the sonic hedgehog gene. Total RNA was extracted from the notochord of a Sprague-Dawley rat at embryonic day 9 and the N-terminal fragment of sonic hedgehog was amplified by nested reverse transcription-PCR. The N-terminal fragment of the sonic hedgehog gene was successfully cloned. The secondary and tertiary structures of the N-terminal fragment of the sonic hedgehog protein were predicted using Jpred and Phyre online.The sonic hedgehog protein not only plays a key role in early embryonic development, but also has essential effects on the adult nervous system, including neural stem cell proliferation, differentiation migration and neuronal axon guidance. The N-terminal fragment of sonic hedgehog is the key functional element in this process. Therefore, this study aimed to clone and analyze the N-terminal fragment of the sonic hedgehog gene. Total RNA was extracted from the notochord of a Sprague-Dawley rat at embryonic day 9 and the N-terminal fragment of sonic hedgehog was amplified by nested reverse transcription-PCR. The N-terminal fragment of the sonic hedgehog gene was successfully cloned. The secondary and tertiary structures of the N-terminal fragment of the sonic hedgehog protein were predicted using Jpred and Phyre online.

关 键 词：neural regeneration basic research sonic hedgehog protein CLONING nested reversetranscription-PCR secondary structure tertiary structure central nervous system developmentalneurobiology rats grants-supported paper photographs-containing paper neuroregeneration 

分 类 号：R318.0[医药卫生—生物医学工程]