PEDF和Avastin对大鼠角膜碱烧伤新生血管作用的比较  

Comparison of inhibitory effects of PEDF and Avastin on alkali-induced corneal neovascularization of rat

作　　者：朱丹[1] 陆晓和[1] 付月[1] 吴伟[1] 陈晓红[1] 

机构地区：[1]南方医科大学珠江医院眼科,中国广东省广州市510282

出　　处：《国际眼科杂志》2013年第2期235-239,共5页International Eye Science

基　　金：广东省科技计划基金(No.2008B080703037);广州市海珠区科技计划基金(No.2010-T-26)~~

摘　　要：目的:比较局部应用PEDF与Avastin对大鼠碱烧伤角膜新生血管(CNV)的作用。方法:建立碱烧伤诱导大鼠CNV模型,将30只SD大鼠随机分为5组:A组于烧伤当日开始给予10μg/mL PEDF点眼;B组于烧伤后第3d开始给予10μg/mL PEDF点眼;C组于烧伤当日开始给予5mg/mL Avastin点眼;D组于烧伤后第3d开始给予5mg/mL Avastin点眼;E组于烧伤当日开始使用生理盐水点眼,均每日4次,每次10μL。裂隙灯显微镜观察CNV情况并计算各组CNV的面积,采用免疫组织化学染色观察VEGF和CD31的表达。结果:第12d时,A组CNV面积小于B组和C组,差异有统计学意义(P<0.01);B,C组与D组相比,差异无统计学意义(P=0.215,0.058)。免疫组织化学检测VEGF及CD31示A组VEGF及CD31表达较弱,B,C,D组表达增多,E组表达显著增强。结论:局部应用Avastin及PEDF均能抑制大鼠角膜化学伤后的CNV。烧伤后早期使用PEDF效果优于Avastin。AIM. To compare the inhibitory effects of PEDF and Avastin on alkali- induced corneal neovascularization （CNV） of rat. METHODS： Thirty eyes of 30 SD rats were chemically cauterized by applying a 3mm- diameter filter paper soaked lmol/L NaOH solution at the central cornea for 40 seconds. All animals were randomly assigned to five groups： group A, the PEDF drops was used the day after cauterization; group B, the PEDF drops was used the third day after cauterization; group C, the Avastin drops was used the day after cauterization; group D, the Avastin drops was used the third day after cauterization; group E, normal saline solution drops was used the day after cauterization （control group）. All the eye drops were applied 101JL every time and 4 times per day. The growth of CNV was observed by slit lamp microscope and the area of CNV was calculated. Expression of VEGF and CD31 was detected by immunohistochemistry on the twelfth day after experiment. RESULTS： On the twelfth day, the area of CNV in group A was smaller than that of group B, C （P〈0.01）. There was no significant difference in terms of CNV areasbetween group B and D （P=0. 215） and between group C and D （P= 0. 058）. Immunohistochemistry revealed that the expression of VEGF and CD31 was little in group A and massive in group E. In group B, C and D, expression of VEGF and CD31 was more than that of group A. CONCLUSION. Topical application of PEDF and Avastin can inhibit alkali- induced CNV, moreover PEDF is superior to Avastin if applied early after cauterization.

关 键 词：角膜新生血管 PEDF AVASTIN 角膜碱烧伤 

分 类 号：R779.1[医药卫生—眼科]