大鼠面神经压榨对面神经元CNTF表达的影响  

Effects of crush injury to the facial nerve on the expressions of CNTF in motoneurons of facial nucleus in rats

作　　者：刘悦[1,2] 李培华[3] 刘稳[3] 神平[3] 许学谷[3] 孙光明[3] 孙海清 

机构地区：[1]徐州医学院研究生学院,江苏徐州221002 [2]常州市第二人民医院耳鼻咽喉科,江苏常州213003 [3]徐州医学院附属医院耳鼻咽喉科,江苏徐州221002 [4]江苏曼迪科技发展有限公司,江苏徐州221000

出　　处：《山东大学耳鼻喉眼学报》2013年第1期19-23,共5页Journal of Otolaryngology and Ophthalmology of Shandong University

基　　金：江苏省2010年度"企业博士集聚计划"资助项目

摘　　要：目的观察将大鼠面神经压榨后面部行为学变化,探索简易稳定的面神经压榨建模方法;检测压榨侧面神经运动神经元中睫状神经营养因子(CNTF)的表达变化。方法选取健康大鼠30只,解剖右侧面神经主干,蚊式血管钳单齿钳夹,持续1 min,设左侧为对照。术后1、7、14、21、35 d,观察大鼠面部行为学改变;HE染色观察面神经运动神经元的病理形态学变化并进行细胞计数;免疫组化观察面神经元CNTF蛋白的表达并进行光密度分析。结果面神经压榨损伤后即刻出现同侧面神经瘫痪,术后7～14 d达高峰,而后逐渐恢复,35 d大鼠面部对称,面瘫完全恢复。HE染色发现术后7、14、21 d损伤侧面神经运动神经元数目较对照组减少(P<0.01),细胞结构形态紊乱,细胞内空泡增多,核仁偏移。免疫组化发现术后7、14、21 d面神经运动神经元CNTF蛋白表达量明显增多(P<0.01)。结论神经压榨是建立可逆性面神经麻痹动物模型简易稳定的方法。面神经压榨损伤后,面神经运动神经元内CNTF蛋白的表达增多。Objective To observe the behavioral difference after the crush injury of facial nerve in rats and determine the expression of CNTF in motoneurons of facial nucleus. Methods In 30 healthy adult rats, the trunk of right facial nerve were crushed while that of the left nerve was control. The rats were divided into 5 subgroups according to the days after the operation, including 1d, 7d, 14d, 21d and 35d subgroup. During the experiment, the behavioral changes were monitored, while the pathological differences of motoneurons were observed by HE staining and the number of cells was counted. CNTF protein in facial nucleus were detected by immunohistochemical method and analyzed by optical density. Results The rats showed right facial palsy immediately after the crush injury. The number of neurons in the right side decreased significantly compared with the left side （P〈0.01） on the 7th, 14th and 21st day. Some morphological changes were observed, such as deranged cellular structure, cytoplasm vacuolization, or nucleolar margination. The expressions of CNTF increased obviously in the right side on the day of 7th, 14th and 21st day （P〈0.01）. Conslusion Nerve crush is a simple, easy and stable way to establish animal model for the reversible facial paralysis. The expression of CNTF protein increases in the motoneurons of facial nucleus after the facial nerve is crushed.

关 键 词：面神经 面神经核 压榨损伤 睫状神经营养因子 

分 类 号：R745.1[医药卫生—神经病学与精神病学]