铜绿假单胞菌NY3所产表面活性剂对原油降解的影响  

Effects of rhamnolipid on oil degradation by Pseudomonas aeruginosa strain NY3

作　　者：常虹[1] 聂麦茜[1] 葛碧洲[1] 刘超[1] 杨琴 周立辉 樊晓宇[1] 孙超[1] 韦绒绒[1] 蒋欣[1] 

机构地区：[1]西安建筑科技大学环境与市政工程学院,西安710055 [2]中国石油长庆油田油气工艺研究院环保研究室,西安710000

出　　处：《环境工程学报》2013年第2期771-776,共6页Chinese Journal of Environmental Engineering

基　　金：陕西省国际科技合作重点项目(2012KW-25);国家自然科学基金面上项目(51278405)

摘　　要：铜绿假单胞菌NY3是从石油污染土壤中分离出的一株能快速代谢疏水性化合物的菌种。研究了该菌产表面活性剂及对原油降解的作用。实验表明,在敞开体系中,投加82 mg/L NY3菌产的鼠李糖脂,240 h能使NY3对原油的降解率提高50%。投加甘油使NY3产鼠李糖脂与降解原油同步进行,与投加鼠李糖脂对原油降解的促进作用相近。投加9‰甘油使NY3在168 h对原油的降解率提高43%。NY3菌能同时降解原油中的直链烷烃及菲(Pr)和芘(Ph)等多环芳烃。在敞开体系中用少量甘油使产鼠李糖脂和降解原油同步进行,节约处理费用。研究结果为NY3菌株在露天石油污染环境修复中的应用奠定了基础。Pseudomonas aeruginosa NY3, being able to metabolize hydrophobic compounds, was isolated from petroleum-contaminated soil samples. Effects of the rhamnolipid and its production on crude oil degradation by NY3 strain were discussed. The results showed that the rhamnolipid produced by NY3 strain could enhance crude oil biodegradation. Comparing with the controls, oil removal rate within 240 h enhanced 50% with 82 mg/ L rhamnolipid. Adding glycerol in NY3 degrading systems, the degradation of crude oil and the rhamnolipid pro- duction took place simultaneously, the instantly produced rhamnolipid also accelerated oil metabolism. Adding 9%e glycerol, the crude oil removal rate by NY3 improved about 43% within 168 h. Phenanthrene （Ph） and py- rene （Pr） containing in the crude oil could also be removed. The cost of the remediation of contaminated water body by NY3 strain can be decreased by using small amount of glycerol, instead of using rhamnolipid directly. The above results lay a foundation for NY3 strain applying to oil-spill clean-up.

关 键 词：鼠李糖脂 铜绿假单胞菌NY3 甘油 原油 生物降解 

分 类 号：X172[环境科学与工程—环境科学]