一起星状病毒引起暴发流行的实验室检测  

An outbreak of Astrovirus and the laboratory detection

作　　者：夏文[1] 倪贤生 吴越[1] 樊国印[1] 周显凤[1] 贺凤兰[1] 

机构地区：[1]南昌市疾病预防控制中心,江西南昌330038

出　　处：《现代预防医学》2013年第4期744-745,共2页Modern Preventive Medicine

摘　　要：目的通过实验室技术手段,确认引起暴发流行的病原微生物,并确立病原微生物载体与病人之间的相关性。方法根据现场流行病学调查提示,对二次供水5份、桶装水2份、蓄水池水1份、病人肛拭子7份,粪便1份,呕吐物1份,运用RT-PCR技术筛查确立可疑病原微生物。结果 5份二次供水、1份蓄水池水均检出人星状病毒核酸,7份病人肛拭子中4份阳性,1份粪便和1份呕吐物也均检出人星状病毒核酸,同时排除人杯状病毒、轮状病毒和腺病毒核酸的存在。结论本次急性胃肠炎暴发流行是由星状病毒污染水源引起的。OBJECTIVE By means of laboratory techniques, to confirm outbreaks caused by pathogenic microorganisms, and the establishment of the correlation between pathogenic microbe and the patients. METHODS We used field epidemiological to investigate five secondary-supply water, 2 bottled water, 1 reservoir water, 7 patients＇ rectal swabs, 1 stool andlvomit sam- ple, and to establish suspicious pathogenic microorganisms with RT-PCR. RESULTS Results of RT-PCR indicated that human astrovirus nucleic acids were detected in five secondary-supply water. 4 out of 7 patients＇ rectal swabs were positive, and 1 stool and lvomit sample too positive. At the same time, human calicivirus, rotavirus and adenovirus were excluded. CON- CLUSION We concluded that the outbreak of acute gastroenteritis was caused by water contaminated by human astrovirus.

关 键 词：星状病毒 急性胃肠炎 RT-PCR 

分 类 号：R57[医药卫生—消化系统]