Biosorption and biodegradation of polycyclic aromatic hydrocarbons by Phanerochaete chrysosporium in aqueous solution  

作　　者：DING Jie CHEN BaoLiang ZHU LiZhong 

机构地区：[1]Department of Environmental Science,Zhejiang University [2]Zhejiang Provincial Key Laboratory of Organic Pollution Process and Control

出　　处：《Chinese Science Bulletin》2013年第6期613-621,共9页

基　　金：supported by the National Natural Science Foundation of China (41071210);the National High Technology Research and Development Program of China (2012AA06A203);Zhejiang Provincial Natural Science Foundation of China (R5100105)

摘　　要：Biosorption and biodegradation of phenanthrene and pyrene by live and heat-killed Phanerochaete chrysosporium are investigated to elucidate the bio-dissipation mechanisms of polycyclic aromatic hydrocarbons(PAHs) in aqueous solution and its regulating factors.The effects of nutrient conditions(carbon source and nitrogen source concentrations),the co-existing Cu 2+,and repeated-batch feed of PAHs on the biosorption and biodegradation are systematically studied.The removal of PAHs by dead bodies of P.chrysosporium is attributed to biosorption only,and the respective partition coefficients of phenanthrene and pyrene are 4040 and 17500 L/kg.Both biosorption and biodegradation contribute to the dissipation of PAHs by live P.chrysosporium in water.After a 3-d incubation,the removal percentage via biosorption are 19.71% and 52.21% for phenanthrene and pyrene,respectively.With the increase of the incubation time(3 40 d),biodegradation gradually increases from 20.40% to 60.62% for phenanthrene,and from 15.55% to 49.21% for pyrene.Correspondingly,the stored-PAHs in the fungal bodies decrease.Under the carbon-rich and nitrogen-limit nutrient conditions,the removal efficiency and biodegradation of phenanthrene and pyrene are significantly promoted,i.e.99.55% and 92.77% for phenanthrene,and 99.47% and 83.97% for pyrene after a 60-d incubation.This phenomenon is ascribed to enhanced-biosorption due to the increase of fungal biomass under carbon-rich condition,and to stimulated-biodegradation under nitrogen-limit condition.For the repeated-batch feed of phenanthrene,the pollutant is continuously removed by live P.chrysosporium,and the contribution of biodegradation is enhanced with the repeated cycles.After 3 cycles,the biodegradation percentage is up to 90% with each cycle of a 6-d incubation.Biosorption and biodegradation of phenanthrene and pyrene by live and heat-killed Phanerochaete chrysosporium are investigat- ed to elucidate the bio-dissipation mechanisms of polycyclic aromatic hydrocarbons （PAHs） in aqueous solution and its regulating factors. The effects of nutrient conditions （carbon source and nitrogen source concentrations）, the co-existing Cu2＋, and repeat- ed-batch feed of PAHs on the biosorption and biodegradation are systematically studied. The removal of PAHs by dead bodies of P. chrysosporium is attributed to biosorption only, and the respective partition coefficients of phenanthrene and pyrene are 4040 and 17500 L/kg. Both biosorption and biodegradation contribute to the dissipation of PAHs by live P. chrysosporium in water. After a 3-d incubation, the removal percentage via biosorption are 19.71% and 52.21% for phenanthrene and pyrene, respectively. With the increase of the incubation time （3-40 d）, biodegradation gradually increases from 20.40% to 60.62% for phenanthrene, and from 15.55% to 49.21% for pyrene. Correspondingly, the stored-PAHs in the fungal bodies decrease. Under the carbon-rich and nitrogen-limit nutrient conditions, the removal efficiency and biodegradation of phenanthrene and pyrene are significantly promoted, i.e. 99.55% and 92.77% for phenanthrene, and 99.47% and 83.97% for pyrene after a 60-d incubation. This phenome- non is ascribed to enhanced-biosorption due to the increase of fungal biomass under carbon-rich condition, and to stimulat- ed-biodegradation under nitrogen-limit condition. For the repeated-batch feed of phenanthrene, the pollutant is continuously re- moved by live P. chrysosporium, and the contribution of biodegradation is enhanced with the repeated cycles. After 3 cycles, the biodegradation percentage is up to 90% with each cycle of a 6-d incubation.

关 键 词：生物降解 生物吸附 多环芳烃 水溶液 白腐菌 耗散机制 营养条件 多环芳香烃 

分 类 号：X13[环境科学与工程—环境科学]