Development of DArT markers for a linkage map of flue-cured tobacco  

作　　者：LU XiuPing GUI YiJie XIAO BingGuang LI YongPing TONG ZhiJun LIU Yun BAI XueFei WU WeiRen XIA Ling HUTTNER Eric KILIAN Adrzej FAN LongJiang 

机构地区：[1]Department of Agronomy,Zhejiang University [2]Yunnan Academy of Tobacco Agricultural Sciences and China Tobacco Breeding Research Center at Yunnan [3]DArT P/L,PO Box 7141,Yarralumla,Canberra,ACT 2600,Australia

出　　处：《Chinese Science Bulletin》2013年第6期641-648,共8页

基　　金：supported by Yunnan Provincial Tobacco Company(08A05//2011YN04);China National Tobacco Company (110200701023/110201201003(JY-03))

摘　　要：Tobacco(Nicotiana tabacum) is one of the most economically important nonfood crops,and flue-cured tobacco accounts for approximately 80% of world tobacco production.An extremely narrow genetic diversity in the tobacco pool has led to a low efficiency of PCR-based molecular markers(such as AFLP and SSR).Diversity Arrays Technology(DArT) is a high-throughput hybridisation-based marker system that has been developed in many plants including wheat,which,like tobacco,has a complex genome.In this study,we developed a tobacco DArT chip that included 7680 representative sequence tags based on typical tobacco accessions.The 1076 DArT markers of flue-cured tobacco were identified and most(82.1%) of their polymorphism information contents(PICs) were greater than 0.4.An integrated linkage map that included 851 markers(238 DArT and 613 SSR),which is the highest density map of flue-cured tobacco to date,was constructed.This chip-based DArT system provides an alternative in high-throughput marker genotyping for tobacco.Tobacco （Nicotiana tabacum） is one of the most economically important nonfood crops, and flue-cured tobacco accounts for ap- proximately 80% of world tobacco production. An extremely narrow genetic diversity in the tobacco pool has led to a low effi- ciency of PCR-based molecular markers （such as AFLP and SSR）. Diversity Arrays Technology （DART） is a high-throughput hybridisation-based marker system that has been developed in many plants including wheat, which, like tobacco, has a complex genome. In this study, we developed a tobacco DArT chip that included 7680 representative sequence tags based on typical to- bacco accessions. The 1076 DArT markers of flue-cured tobacco were identified and most （82.1%） of their polymorphism infor- mation contents （PICs） were greater than 0.4. An integrated linkage map that included 851 markers （238 DArT and 613 SSR）, which is the highest density map of flue-cured tobacco to date, was constructed. This chip-based DArT system provides an al- ternative in high-throughput marker genotyping for tobacco.

关 键 词：DART 分子标记 连锁图谱 烤烟 烟草生产 遗传多样性 多态信息含量 标记系统 

分 类 号：TS452[农业科学—烟草工业]