光照对月季‘光谱’花青素合成及其CHS和DFR基因表达的影响  

Effect of Light on the Anthocyanin Biosythesis and Expression of CHS and DFR in Rosa chinensis ‘Spectra’

作　　者：骆菁菁[1] 李虹[1] 柏斌斌[1] 俞红强[1] 游捷[1] 

机构地区：[1]中国农业大学农学与生物技术学院,北京100193

出　　处：《分子植物育种》2013年第1期126-131,共6页Molecular Plant Breeding

基　　金：农业部948重点项目(2008-G3);农业部行业科技项目(200903020)共同资助

摘　　要：探明变色月季花开放过程中花色变化的相关调控机制,可以为月季花色育种提供理论依据。本研究测定了月季‘光谱’在正常光照和遮光条件下的总花青素含量及月季‘光谱’花冠中CHS、DFR基因在两种条件下的差异表达,试图同时从代谢水平以及分子水平上进行探讨变色月季‘光谱’的变色机理。研究结果表明:光照是影响‘光谱’变色的关键环境因素,遮光阻碍了‘光谱’花瓣中花青素的合成,抑制了基因CHS和DFR的表达,导致花冠不着色,证明了花青素是决定月季的花冠由黄色变为红色的主要物质,也证明了基因CHS和DFR在花青素合成过程中的重要作用。Figuring out the possible regulatory mechanisms of flower color changing during the flowering stage of Chinese rosemight provide genetically insight for the color breeding of roses. This study was carried out at the metabolic and molecular level to measure total contents of anthocyanin and carotenoid as well as determine and the differential expression of CHS and DFR genes of the rose cultivar ‘spectra’ under the light and shading conditions in order to explore the regulatory mechanism at the metabolic level and molecular level. The results showed that lighting was the key to effect the color change of rose cultivar ‘spectra’. The shading condition hinders the synthesis of anthocyanin in the petals of ‘spectra’, as well as inhibits the expression of genes CHS and DFR, which caused that the corolla couldn＇t colored. These results proved that the genes CHS and DFR play important roles in the process of anthocyanin synthesis, and also proved that anthocyanine is one of the factors that may determine the color changes of rose petals from yellow to red.

关 键 词：月季 月季品种'光谱’ 花青素 基因表达量 

分 类 号：S685.12[农业科学—观赏园艺]