纤溶酶对兔后发性白内障的抑制作用  

The inhibitory effect of plasmin on posterior capsule opacity

作　　者：张筠[1] 程亚辉[1] 闫志鹏[1] 赵平[1] 

机构地区：[1]河北医科大学第三医院眼病科,石家庄050051

出　　处：《中华眼视光学与视觉科学杂志》2013年第1期41-44,共4页Chinese Journal Of Optometry Ophthalmology And Visual Science

基　　金：河北省2011年医学科学研究重点课题计划项目（20110109）

摘　　要：目的探讨纤溶酶对后发性白内障（PCO）形成的抑制作用机制。方法实验研究。将20只健康4月龄新西兰大白兔随机分为对照组和实验组，每组各10只。两组均行晶状体超声乳化摘除术，术毕对照组给予0．2m10．9％氯化钠注射液前房内注射，实验组给予注射用纤溶酶1IU前房注射。分别于术后1、3、7、14、30、60d进行眼部裂隙灯检查，观察前房炎症反应、PCO的发生时间及手术后60d时PCO发生率：采用ELISA检测房水中碱性成纤维细胞生长因子（bFGF）含量；应用计算机图像分析技术对免疫组化染色的晶状体上皮细胞增殖细胞核抗原（PCNA）的表达进行半定量分析。数据分析采用卡方检验和成组t检验。结果①术后前房炎症：术后第1天，实验组和对照组的所有术眼均出现不同程度前房炎症反应：术后第3天，前房炎症反应开始消退，术后3、7d实验组炎症发生率均较对照组低，差异有统计学意义（x2=4．33，P〈0．05；X2=7．06，P〈0．01）。②.PCO发生率：术后60d时实验组为45％．对照组为90％．两组差异有统计学意义（X。=6．14，P〈0．05）。（幽FGF的含量：术后1、3、7、14、30d，实验组bFGF含量较对照组低，差异有统计学意义（t=70．77，70．35，46．77，46．69，14．22，P均〈0．01）；60d时两组间差异无统计学意义（t=1．93，P〉O．05）。PCNA表达：实验组后囊膜可见单层细胞染色，对照组后囊膜可见多层细胞染色。实验组与对照组积分光密度值差异有统计学意义（t=23．99，P〈0．01）。结论①前房注射纤溶酶可以有效地减轻前房炎症反应并且促进炎症的恢复。②纤溶酶可以降低前房内bFGF的含量。③纤溶酶可以有效地降低PCO的发生率和延缓其发展速度。Objective To investigate the inhibitory mechanism of plasmin on the formation of posterior capsule opacity （PCO）. Methods In an experimental study, twenty 4-month-old New Zealand white rabbits were selected （approximate weight 2.0 to 2.5 kg）. No eyeelated pathological defects were observed in any rabbits. They were randomly divided into two groups of 10 rabbits each： a control group and an experimental group. All rabbits underwent phacoemulsification surgery under general anesthesia. At the conclusion of the surgery, rabbits in the experimental group were injected with plasmin 1 IU （0.2 ml） in the anterior chamber and the control group was injected with saline 0.2 ml. Inflammation in the anterior chamber and the mobidity of PCO were recorded over a period of time. Basic fibroblast growth factor （bFGF） was measured by the ELISA method for each group. In addition, a semi-quantitative analysis of lens proliferating cell nuclear antigen （PCNA） was performed by immunohistochemical staining using a computer assisted image analysis system. All data were analyzed by the SPSS 13.0 statistical software program （P〈0.05 was considered as a significant difference）. Results @Inflammation of the anterior chamber （AC）. On the first day after surgery, all rabbit eyes in the two groups presented with variable inflammation of the AC. After 3 days, reaction in the AC was reduced and the morbidity of the inflammation was significantly decreased in the experimental group compared to the control group at 3 days and 7 days （X2=4.33, P〈0.05; X2= 7.06, P〈0.01）. Morbidity of. PCO. The morbidity of the PCO was 45% in the experimental group and 90% in the control group at 60 days. The decrease was significant compared to the control group（Xz=6.14, P〈0.05）. Concentration of basic fibroblast growth factor （bFGF）. ELISA analysis demonstrated that the concentration of bFGF in the experimental group was significantly decreased compared to the control group at all time points from one day to 4 week

关 键 词：纤溶酶 后发性白内障 碱性成纤维细胞生长因子 增殖细胞核抗原 

分 类 号：R77[医药卫生—眼科]