改良大鼠肺挫伤模型的制作方法  

A method for establishment of rat model with pulmonary contusion

作　　者：王邵华[1] 王晋[1] 陈熹[1] 阮征[1] 张洁[2] 郑瑾[3] 

机构地区：[1]上海交通大学附属第一人民医院胸外科,200433 [2]上海交通大学附属第一人民医院放射科,200433 [3]上海交通大学附属第一人民医院病理科,200433

出　　处：《中华创伤杂志》2013年第2期180-184,共5页Chinese Journal of Trauma

摘　　要：目的建立一种简便、特异的大鼠单纯双肺挫伤模型，并确定最大亚致死损伤能量。方法以自由落体砝码产生能量，通过特制的心前区保护平板将能量传导至大鼠双侧后胸壁。根据不同能量分为4组：2．1，2．4，2．7，3．0J组。伤后4h，通过动脉血气分析、三维计算机成像（3DCT）测定肺挫伤占双肺容积百分比评价肺挫伤后肺损伤程度，并行肺、心肌病理学检查确诊肺挫伤和排除心肌挫伤。结果损伤能量3．0J组大鼠死亡率高达33％，2．4J组死亡率11％；2．7J组PaO：明显低于2．4J组（P〈0．01），肺挫伤容积百分比明显高于2．4J组（P〈0．01）；各组PaO，与3DCT肺挫伤容积百分比均呈负相关（R^2=0．762）。肺活检可见出血、肺不张及中性粒细胞浸润。心肌活检未见明显肌纤维断裂等变化。结论本研究方法可复制满意的单纯双肺挫伤模型，2．7J可视为本模型的最大亚致死损伤能量。Objective To create an easy and specific rat model of isolated bilateral pulmonary contusion and determine the maximal sublethal injury energy. Methods Injury energy was produced by free falling weights and passed through a designed precordial shield to rats＇ bilateral posterolateral chest wall. The rats were divided into 2.1 J, 2.4 J, 2.7 J and 3.0 J groups, according to the volume of injury energy. Percentage of lung contusion volume in bilateral lung was measured by blood gas analysis and three dimensional CT （3DCT） at four hours post-injury to assess lung injury severity after contusion. Pathological examination of heart and lung tissue was performed to confirm pulmonary contusion and rule out myocardial contusion. Results Death rate in 3.0 J and 2.4 J groups was 33% and 11% , respectively. PaO2 in 2.7 J group was significantly lower than that in 2.4 J group （ P 〈 0.01 ） , but pulmonary contusion percentage in 2.7 J group was significantly higher than that in 2.4 J group （ P 〈 0.01）. All groups showed negative correlation between PaO2 and pulmonary contusion percentage measured by 3DCT （R2 = 0. 762 ）. Hemorrhage, atelectasis and neutrophil infiltration were documented in lung biopsy. No evidence of myofiber break was recorded in heart biopsy. Conclusion This method can duplicate satisfactory models of isolated bilateral pulmonary contusion and 2.7 J can be regarded as the maximal sublethral injury energy.

关 键 词：肺 挫伤 成像 三维 

分 类 号：R-332[医药卫生]