纳米活性炭吸附多烯紫杉醇对A549细胞增殖及凋亡的影响  

Effects of docetaxel-loaded activated carbon nanoparticles on proliferation and apoptosis of A549 cells

作　　者：蔡要欣[1,2] 张韧铮[2] 孙岚[1] 宋东颖[1,3] 刘贵富[4] 张莉蓉[2] 张英鸽[1] 

机构地区：[1]军事医学科学院毒物药物研究所 [2]郑州大学基础医学院药理学教研室 [3]吉林大学药学院 [4]中国人民解放军66018部队卫生队

出　　处：《郑州大学学报（医学版）》2012年第6期759-761,共3页Journal of Zhengzhou University(Medical Sciences)

基　　金：国家863计划项目2006AA03Z333;国家973计划项目2010CD933904

摘　　要：目的:研究纳米活性炭吸附多烯紫杉醇(ACNP-DOC)对人肺腺癌细胞A549增殖及凋亡的影响。方法:将A549细胞分别用0.2、1.0、5.0、25.0和125.0μmol/LDOC和ACNP-DOC培养12、24、48和72h后,MTT法测细胞增殖抑制率。将A549分别用IC50浓度的ACNP-DOC、DOC和与ACNP-DOC等量的ACNP培养24h后,应用流式细胞仪检测细胞凋亡率。结果:0.2～125.0μmol/L的DOC及ACNP-DOC对A549细胞均有增殖抑制作用,作用呈浓度和时间依赖性,ACNP-DOC组细胞增殖抑制率高于DOC组(F药物=12.326,F时间=76.247,F浓度=53.028,F交互=20.842,P<0.05)。ACNP-DOC的IC50为0.80μmol/L,DOC为43.45μmol/L。ACNP、DOC和ACNP-DOC组的细胞总凋亡率差异有统计学意义(F=30.208,P<0.001),ACNP-DOC组凋亡率高于ACNP组和DOC组(P均<0.001)。结论:ACNP-DOC较DOC对A549细胞有较强的增殖抑制及诱导凋亡作用。Aim：To study the effects of docetaxel（DOC）-loaded activated carbon nanoparticles（ACNP-DOC） on proliferation and apoptosis of human lung cancer A549 cells.Methods：A549 cells were teated with 0.2,1.0,5.0,25.0 and 125.0 μmol/L DOC or ACNP-DOC for 12,24,48 and 72 h,respectively,then the proliferation inhibition rate was assayed by MTT.A549 cells were teated with ACNP-DOC,DOC and ACNP at dose of IC50 for 24 h,then the apoptosis rate was detected by flow cytometry.Results：DOC and ACNP-DOC in the concentration range of 0.2~125.0 μmol/L could inhibit A549 cell proliferation in a time-and concentration-dependent manner,but the effects of ACNP-DOC were significantly stronger（Fdrug=12.326,Ftime=76.247,Fdose=53.028,Finteraction=20.842,P0.05）.IC50 of ACNP-DOC and DOC was 0.80 and 43.45 μmol/L,respectively.The apoptosis rate of A549 cells treated with ACNP,DOC,or ACNP-DOC at the dose of IC50 differed from each other（F=30.208,P0.001）,and that of ACNP-DOC group was higher than those of ACNP group and DOC group（P0.001）.Conclusion：ACNP-DOC can inhibit A549 cell proliferation,induce apoptosis,and the effects is stronger than DOC.

关 键 词：纳米活性炭 多烯紫杉醇 A549细胞 凋亡 增殖 

分 类 号：R734.2[医药卫生—肿瘤]