癫痫鼠痫性皮层及正常鼠脑皮层组织基因的差异表达研究  

Study on The different Expression Genes between The Epilepsy Rats and The Normal Rats' Brain Issue

作　　者：向军[1] 

机构地区：[1]中南大学湘雅二医院神经外科,长沙410011

出　　处：《中国医药导刊》2012年第12期2167-2168,共2页Chinese Journal of Medicinal Guide

摘　　要：目的:探究癫痫鼠痫性皮层及正常鼠脑皮层组织基因的差异。方法:本实验将40只大鼠随机分为癫痫组与对照组2组,其中电刺激一侧杏仁核点燃20例作为模型组,放置刺激电极但未予电刺激点燃(假手术组)20例作对照组。采用DDRT-PCR技术研究癫痫鼠痫性皮层和正常鼠脑皮层组织基因的差异。结果:研究癫痫鼠痫性皮层和正常鼠脑皮层组织基因的差异,从中发现了7个差异基因.Timm23,NGF-IA,Fbx38_predicted,ATPase synthase,subunit 6,APP,PAF-AH beta,NG5 protein。其中3个基因高表达,4个基因低表达。结论:采用DDRT-PCR技术有助于快速发现了癫痫鼠痫性皮层和正常鼠脑皮层组织差异的7个差异基因,这些基因有可能与癫痫产生有关。Objective：Study on the difference of the genes between the epileptic rats and the normal ratsapos;cerebral cortex tissues.Methods：In this experiment,40 rats were randomly divided into 2 groups：the epilepsy group and the control group.The 20 cases of electrical stimμlation of the unilateral of the amygdala were considered as the model group.Another 20 cases placed a stimμlation electrode but without electrical kindling stimμlations（sham operation group） were served as the control group.By using DDRT-PCR technology we studied the deferent expression of the genes between the epilepsy ratsapos;epileptic cortex and normal ratsapos;cerebral cortex tissue.Results：The study found 7 different genes.They are Timm23,NGF-IA,Fbx38 predicted,ATPase synthase,subunit 6,APP,PAF-AH beta,NG5 protein.Three genes show high expression.Four genes show low expression.Conclusion：The DDRT-PCR technique contributes to the rapid discovery of the deferent expression of seven genes epilepsy ratsapos;epileptic cortex and normal ratapos;s cerebral cortex.These genes has the relation with the epilepsy rats.

关 键 词：癫痫鼠 痫性皮层 脑皮层组织 

分 类 号：R742.1[医药卫生—神经病学与精神病学]