荧光原位杂交与免疫组织化学方法对乳腺癌Her-2检测的比较  

Comparison of Her-2 Detections by Fluorescence in situ Hybridization(FISH) and by Immunohistochemistry(IHC) in Breast Cancer

作　　者：徐国森[1] 林海峰[1] 佟丹丹[1,2] 耿敬姝[1] 

机构地区：[1]哈尔滨医科大学附属第三医院,黑龙江哈尔滨150086 [2]哈尔滨医科大学病理教研室,黑龙江哈尔滨150086

出　　处：《肿瘤学杂志》2012年第12期939-943,共5页Journal of Chinese Oncology

基　　金：黑龙江省自然基金(D200921);哈尔滨医科大学附属第三医院院内基金

摘　　要：[目的]探讨荧光原位杂交(FISH)与免疫组织化学方法(IHC)检测乳腺癌组织中人类表皮生长因子受体2(Her-2)表达结果的差异。[方法]采用FISH和IHC法分别检测508例乳腺癌标本中Her-2基因扩增和Her-2蛋白表达状况。[结果]在IHC法检测Her-2蛋白表达0/+的104例(20.47%)标本中,FISH检出13例(2.56%)Her-2基因扩增,89例(17.52%)为非扩增,2例(0.39%)为可疑;Her-2蛋白表达++的245例(48.23%)标本中,FISH检出121例(23.82%)Her-2基因扩增,120例(23.62%)为非扩增,4例(0.79%)为可疑;IHC在Her-2蛋白表达+++的159例(31.30%)标本中,FISH检出137例(26.97%)Her-2基因扩增,22例(4.33%)为非扩增。[结论]对于IHC法初筛Her-2蛋白为++～+++的患者必须进行FISH检测,以明确Her-2基因的状态,从而指导临床医师诊断治疗。[Purpose ] To investigate the difference of Her-2 expression detected by fluorescence in situ hybridization （FISH） and by immunohistochemistry （IHC） in breast cancer. [Methods] Both IHC and FISH techniques were performed to detect Her-2 gene amplification and Her-2 protein expression respectively in 508 samples with breast cancer. [Results] Among 104 cases （20.47%） with 0/＋ Her-2 protein expression detected by IHC, 13 cases（2.56%） were Her-2 gene amplification detected by FISH;89 cases （17.52%）,non-amplification; 2 cases （0.39%）,suspi- cious. Among 245 cases （48.23%） with ＋＋ Her-2 protein expression, 121 cases （23.82%） were Her-2 gene amplification;120 cases（23.62%） , r^on-amplification; 4 cases（0.79%）, suspicious. A- mong 159 cases （31.30%） with ＋＋＋ Her-2 protein expression, 137 cases （2.97%） were Her-2 gene amplification;22 cases（4.33%） ,non-amplification. [Conclusion] Detecting the amplifica- tion of Her-2 gene with FISH may be helpful to know Her-2 gene status for treatment decision in breast cancer patients with positive Her-2 expression ＋＋～＋＋＋ by IHC.

关 键 词：乳腺肿瘤 荧光原位杂交 人类表皮生长因子受体 免疫组织化学 多倍体 

分 类 号：R737.9[医药卫生—肿瘤]