APC基因启动子区甲基化及环境因素与前列腺癌的关系  

Relationship of methylation status in promoter of APC gene and environmental factors with prostate cancer

作　　者：邱镇[1] 张连升[1] 梁永 崔飞伦[1] 

机构地区：[1]江苏大学附属人民医院泌尿外科,212002 [2]兖州市九一医院泌尿外科

出　　处：《江苏医药》2013年第2期150-153,共4页Jiangsu Medical Journal

基　　金：江苏省自然科学基金(BK2011483)

摘　　要：目的探讨腺瘤样结肠息肉易感基因(APC)启动子区CpG甲基化及环境危险因素及相互作用与中国人群中前列腺癌(PCa)发病之间的关系。方法应用亚硫酸盐修饰后测序法检测60例PCa及40例良性前列腺增生(BPH)组织中APC基因启动子区CpG甲基化情况。分析APC基因甲基化及环境危险因素与PCa患病之间的关系。结果 APC基因在PCa和BPH组织中CG位点甲基化率分别为48.84%和1.19%;基因甲基化率与前列腺特异性抗原(PSA)、Gleason评分、病理分期和PCa危险分期之间关系密切;饮茶者患PCa的危险性是不饮茶者的0.29倍。结论APC基因启动子区甲基化与PCa发生及发展有关;其甲基化率的变化与PCa的危险分期关系密切。检测前列腺组织中相关基因甲基化状态,有望成为诊断早期PCa的一种方法。Objective To investigate the relationship of methylation status in promoter of adenomatosis polyposis coli（APC） gene and environmental factors with prostate cancer（PCa）. Methods With bisulphite sequencing PCR,the methylation of APC gene was detected in PCa tissues of 60 PCa patients and hyperplastic tissues of 40 patients with benign prostate hyperplasia（BPH）.The methylation status and environmental risk factors for PCa were analyzed. Results The methylation rates of CG sites in promoter of APC gene in PCa and BPH tissues were 48.84% and 1.19%,respectively.The methylation rate of APC gene was closely associated with prostate specific antigen（PSA）,Gleason score,pathologic stage and classification of the risks. A decreased risk for PCa was observed in Chinese population having a tea drinking habit. Conclusion The methylation of promoter in APC genes is associated with the occurrence and progress of PCa.The change of methylation rate of APC genes is closely related to the classification of PCa risks.Detection of the methylation status of APC genes may hopefully become an indicator for early diagnosis of PCa.

关 键 词：前列腺癌 腺瘤样结肠息肉易感基因 

分 类 号：R737[医药卫生—肿瘤]