神经生长因子在原发性肝癌中的表达及其临床意义  

Expression and clinical significance of nerve growth factor in primary liver cancer

作　　者：黎培员[1] 柯小丽[2] 朱倩[1] 田德安[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院消化内科,武汉430030 [2]北京市普仁医院消化内科

出　　处：《中华肝脏病杂志》2013年第2期121-124,共4页Chinese Journal of Hepatology

基　　金：国家自然科学基金（81001066,81070333）;高等学校博士学科点专项科研基金（20070248082）

摘　　要：目的观察神经生长因子（NGF）在原发性肝癌组织中的表达及在患者血清中的含量，了解血清NGF水平与肝癌临床病理特征的关系。方法26例肝细胞癌及对应癌旁组织，采用半定量PCR、免疫印迹及免疫组织化学分析NGFmRNA和蛋白的表达及其分布。健康对照者、慢性肝炎、肝硬化及肝癌患者共150例，采用酶联免疫吸附法（ELISA）检测血清NGF的水平，分析其临床意义。两组之间差异的显著性比较用f检验、Mann-WhitneyU检验，多组之间差异的显著性比较应用Kmskal-WauisH检验。数据均采用SPSS11．5统计软件处理。结果肝癌组织中NGF在mRNA及蛋白水平的表达高于癌旁组织，NGF主要分布于肝细胞胞质。血清NGF水平：肝癌组、肝硬化组、慢性肝炎组患者和正常对照组分别为（33．86±16．11）pa／ml、（20．57士9．73）pg／ml、（13．20±6．23）pg／ml和（11．13±6．12）pg／ml，肝癌组、肝硬化组显著高于慢陆肝炎组患者和正常对照组，x^2值分别为39．119和12．041，P值均〈0．0l。肿瘤直径大于5cm及TNM分级为Ⅲ、IV级的肝癌患者血清NGF的水平高于肿瘤直径小于5cm及TNM分级为I、Ⅱ级的患者。结论NGF在肝癌组织中高表达，肝癌患者血清NGF水平明显升高，与肿瘤体积和TNM分级相关，提示NGF在肝癌的发生和发展过程中可能发挥一定作用，血清NGF有助于肝癌患者的病情评估和预后估计。Objective To determine the expression of nerve growth factor （NGF） in hepatic tissues and serum of patients with primary liver cancer （PLC）, and to investigate the relationship of serum NGF levels with clinicopathological features of PLC. Methods Hepatocellular carcinoma （HCC） samples and patient-matched tumor-adjacent liver samples were collected from 26 PLC patients to assess the mRNA and protein expressions of NGF by reverse transcription-PCR, western blotting （b-actin normalized）, and immunohistochemistry. In addition, serum samples were collected from 40 PLC patients, 40 liver cirrhosis patients, 40 chronic hepatitis patients （including hepatitis B or C virus infections）, and 30 healthy （normal） controls. The serum levels of NGF were measured by enzyme-linked immunosorbent assay. Intergroup differences were assessed by the t-test, and correlation with sex, age, presence of cirrhosis, tumor size, and TNM classification were assessed by the Kruskal-Wallis H test followed the Mann-Whitney U test. Results HCC tissues showed higher mRNA and protein expressions of NGF than the corresponding tumor-adjacent non-HCC tissues. Hepatic NGF expression was mainly localized to the tumor cell cytoplasm. Serum NGF expression was significantly higher in PLC patients （33.86±16.11 pg/ml） and cirrhosis patients （20.57±9.73 pg/ml） than in normal controls （ 11.13±6.12 pg/ml） and chronic hepatitis patients （13.20±6.23 pg,/ml） （P 〈 0.01 ）.Furthermore, when the PLC patients were stratified according to tumor size and TNM stage, the serum NGF level was found to be significantly higher in patients with tumors 〉 5 cm （vs. 〈 5 cm; U = 83.000, P = 0.002） or of TNM stage IIIflV （vs. stage I/II; U= 103.500, P = 0.009）. Conclusion Elevated expression of NGF in liver cancer tissues and serum of PLC patients is related with tumor size and TNM staging. These findings suggest that NGF may play a role in HCC tumorigenesis and/or that serum NGF may represent a prognostic marker of PLC.

关 键 词：癌 肝细胞 神经生长因子 预后 评价研究 

分 类 号：R735.7[医药卫生—肿瘤]