重复性经颅磁刺激与舍曲林改善CUMS大鼠焦虑抑郁样行为的比较及其机制研究  

The comparison of rTMS and sertraline ameliorate anxiety-and depression-like behaviors in a rat model of CUMS and the possible mechanism

作　　者：王磊[1] 陈怡环[1] 杨帆[1] 彭正午[1] 谭庆荣[1] 

机构地区：[1]第四军医大学西京医院心身科,西安710032

出　　处：《神经解剖学杂志》2013年第1期65-69,共5页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金;81171285

摘　　要：目的:比较重复性经颅磁刺激(repetitive transcranial magnetic stimulation,rTMS)和舍曲林处理对慢性不可预见温和应激(chronic unpredictable mild stress,CUMS)大鼠焦虑抑郁样行为的改善作用及其可能的作用机制。方法:32只SD大鼠随机分为对照组、CUMS组、CUMS+rTMS组以及CUMS+舍曲林组,每组8只。采用糖水偏好实验、强迫游泳实验及旷场实验观察各组大鼠行为,并以蛋白质印迹法(Western blot)检测各组大鼠海马内源性大麻素I型受体(cannabinoid type 1 receptor,CB1R)的表达。结果:CUMS组糖水偏好值、水平活动度、中央活动次数以及CB1R的表达水平均显著低于对照组、CUMS+rTMS组和CUMS+舍曲林组,而CUMS+rTMS组和CUMS+舍曲林组两者之间没有统计学差异;CUMS组的强迫游泳不动时间大于其它三组,而CUMS+rTMS组和CUMS+舍曲林组两者之间没有统计学差异。结论:rTMS与舍曲林均能改善CUMS大鼠的焦虑抑郁样行为,二者的改善作用没有统计学差异,其作用可能是通过上调海马CB1R的表达实现的。Objective： To compare the effects and the underlying mechanisms of repetitive transcranial magnetic stimula- tion （rTMS） and sertratline administration in ameliorating anxiety- and depression-like behaviors in a rat model of chronic unpredictable mild stress （CUMS） . Methods ： 32 adult male SD rats were randomly divided into control, CUMS, CUMS ＋ rTMS and CUMS ＋ sertraline groups （ n = 8 for each group）. The behavior of rats were investigated by sucrose prefer- ence test, forced swimming test and open-field test, and the expression of cannabinoid receptor type 1 receptor （CB1R） in hippocampus was measured by Western blot. Results： The sucrose preference percentage, horizontal motion distance, the number of entering central region and the expression of CB1R in CUMS group was less than those in control, CUMS ＋ rTMS and CUMS ＋ sertraline groups, but there was no significant difference between CUMS ＋ rTMS group and CUMS ＋ sertraline group; the immobility time in CUMS group was longer than that in other three groups, but there was no signifi- cant difference between CUMS ＋ rTMS group and CUMS ＋ sertraline group. Conclusion： Both rTMS and sertraline can improve anxiety and depression behaviors of CUMS rats with no significant difference between the two, the effects of rTMS and sertralin may be mediated by up-regulation of the expression of CB1 R.

关 键 词：重复性经颅磁刺激 舍曲林 慢性不可预见温和应激模型 内源性大麻素I型受体 大鼠 

分 类 号：R749.4[医药卫生—神经病学与精神病学]