抗VEGFR-2嵌合Fab抗体对裸鼠肝癌原位移植瘤新生血管生成的影响  

Effect of a Chimeric Anti-VEGFR-2 Fab Antibody on Angiogenesis of an Orthotopic Xenograft Tumor of Hepatocellular Carcinoma in Mice

作　　者：李倩君[1] 周传文[1] 王丙剑[1] 朱进[2] 张建民[3] 吴亚夫[3] 潘峰[1] 

机构地区：[1]南京医科大学附属淮安第一医院,江苏淮安223300 [2]南京军区军事医学研究所,江苏南京210002 [3]南京医科大学鼓楼临床医学院肝胆外科,江苏南京210008

出　　处：《现代生物医学进展》2012年第34期6643-6646,共4页Progress in Modern Biomedicine

基　　金：南京医科大学科技发展基金重点项目(2011NJMU241)

摘　　要：目的:检测抗VEGFR-2嵌合Fab抗体对裸鼠肝癌原位移植瘤血管生成的影响。方法:建立裸鼠肝癌H22细胞原位移植瘤模型,随机分成生理盐水组(n=12)和抗体组(n=12)。采用免疫组织化学SP染色法对两组肝脏移植瘤进行血管染色,观察其微血管密度(MVD)情况。结果:成功建立裸鼠H22肝癌原位移植瘤模型,HE染色显示肝脏移植瘤为肝细胞肝癌,免疫组化结果显示肝脏实体瘤内微血管密度抗体组较生理盐水组显著性减少(25.64±1.53 vs 8.65±1.79,P<0.05)。结论:抗VEGFR-2嵌合Fab抗体能够抑制裸鼠肝癌原位移植瘤的血管生成。Objective： To investigate the effect of a chimeric anti-VEGFR-2 Fab antibody on angiogenesis of an orthotopic xenograft tumor in nude mice.Methods： An orthotopic xenograft tumor model of hepatocellular carcinoma was created by injection of H22 cells directly into the liver parenchyma of nude mice,and twenty-four nude mice bearing orthotopic HCC were randomly divided into two groups： saline group（n=12）,antibody group（n=12）.The microvessel density in H22 solid tumor of the two groups was counted by SP staining of immunohistochemistry.Results： An orthotopic xenograft tumor model of hepatocellular carcinoma was successfully created and was showed as hepatocellular carcinoma by hematoxylin-eosin staining.The microvessel density in antibody group was significantly lower than that in saline group（25.64±1.53 vs 8.65±1.79,P0.05） by immunohistochemistry.Conclusions： The chimeric anti-VEGFR-2 Fab antibody can inhibit angiogenesis of an orthotopic xenograft tumor in nude mice.

关 键 词：肝肿瘤 动物模型 嵌合Fab抗体 血管内皮生长因子受体-2 

分 类 号：Q95-3[生物学—动物学] R735.7[医药卫生—肿瘤]