低蛋白配伍α酮酸饮食对糖尿病大鼠肾组织 TGF-β及其受体表达的影响  

Effects of low protein diet with α-keto acid supplemented on gene expression of TGF-β and its receptors in diabetic rats

作　　者：杨秀[1] 王鸣[1] 费晓[1] 屠巧峰[1] 余道军[1] 谢祥成[1] 马立彬[1] 

机构地区：[1]杭州市第一人民医院肾内科,310006

出　　处：《浙江医学》2012年第23期1877-1879,1888,共4页Zhejiang Medical Journal

基　　金：杭州市医学重点专科专病项目（20091233Q07）

摘　　要：目的探讨低蛋白配伍α酮酸饮食对糖尿病大鼠肾组织 TGF-β及其受体表达的影响.方法将实验大鼠随机分为：健康大鼠对照组[对照组,给予正常蛋白饮食（含18.5%酪蛋白）]、糖尿病模型组（NPD 组,给予正常蛋白饮食）;糖尿病模型低蛋白饮食组[LPD 组,给予低蛋白饮食（含6%酪蛋白）];糖尿病模型低蛋白配伍α酮酸饮食组[LK 组,给予低蛋白配伍α酮酸饮食（含5%酪蛋白＋1%α酮酸）].每组8只,饲养12周.收集血、尿标本,检测生化指标及24h 尿蛋白定量;取肾组织标本,实时定量 PCR 法检测 TGF-β1及Ⅰ型受体（TβRI）、Ⅱ型受体（TβRⅡ）mRNA 水平.结果 NPD 组 Scr、BUN 及24h 尿蛋白定量水平均明显高于LPD 组和 LK 组（均 P〈0.05）.LPD 组和 LK 组肾组织 TGF-β1及 TβRI、TβRⅡ mRNA 基因表达水平均较 NPD 组明显下调（均 P〈0.05）,且 LK 组较 LPD 组下调更显著（P〈0.05）.结论低蛋白配伍α酮酸饮食可能通过下调肾组织 TGF-β1及其受体 TβRI、TβRⅡ mRNA 基因表达水平保护肾功能,减少蛋白尿。Objective To investigate the effects of low protein diet with α-keto acid supplemented on renal expression of TGF-β and its receptors type I （TβRI） and type Ⅱ（TβRⅡ） mRNA in diabetic rats. Methods Diabetes was induced in 24 male SD rats, then the animals were randomly assigned to three groups with 8 in each group： normal protein group （NPD, fed with 18.5% casein protein）, low protein group （LPD, fed with 6% casein protein） and low protein with α-keto acid supplemented group （LK, fed with 5% casein protein＋1% α-keto acid）. The rats were sacrificed at the end of 12th week, serum creatinine, blood urea, serum albumin and 24-h urinary protein excretion were measured. Real-time PCR was used to detect the mRNA ex-pression of TGF-β1, TβRI and TβRII. Results Serum creatinine, blood urea and proteinuria of NPD group were significantly higher than those of LPD and LK groups（P〈0.05）. Expression of TGF-β1, TβRI and TβRⅡmRNAs in LPD and LK groups were significantly lower than those in NPD group（P〈0.05）, and those in LK group were lower than in LPD group（P〈0.05）. Conclu-sion Low protein diet with α-keto acid supplemented exhibits renal protective effects, which might be related to the down-regulating expression of TGF-β1 and its receptors.

关 键 词：转化生长因子Β 糖尿病肾病低蛋白饮食 Α酮酸 

分 类 号：R575.2[医药卫生—消化系统]