ABCB1基因多态性与肾移植受者环孢素所致肝功能异常的相关性研究  

Association of ABCB1 Genetic Polymorphism with Cyclosporine-induced Liver Injury in Chinese Renal Transplant Recipients

作　　者：辛华雯[1] 刘慧明[1] 李元启 黄晖 赵丽 余爱荣[1] 李罄[1] 吴笑春[1] 李维亮[1] 熊磊[1] 

机构地区：[1]广州军区武汉总医院临床药理科,湖北武汉430070 [2]湖北省药品(医疗器械)不良反应监测中心

出　　处：《华南国防医学杂志》2013年第1期17-20,F0003,共5页Military Medical Journal of South China

基　　金：湖北省自然科学基金项目(2009CDB010;2011CDC074)

摘　　要：目的研究肾移植术后患者ABCB1基因多态性对环孢素A(cyclosporin A,CsA)所致肝功能异常的影响。方法采用聚合酶链反应(polymerase chain reaction,PCR)-限制性片段长度多态性(restriction fragment lengthpolymor-phism,RFLP)方法(PCR-RFLP),对339名服用CsA的肾移植患者进行ABCB1基因1236C>T、2677G>T/A和3435C>T多态性检测,根据肾移植患者发生肝脏损伤的情况分为CsA致肝功异常组、其他原因肝损伤组和对照组。结果在339名肾移植患者中,ABCB1基因1236C>T、2677G>T/A和3435C>T突变等位基因频率分别为64.25%、51.54%和35.75%。ABCB1基因1236C>T、2677G>T/A和3435C>T多态性在CsA致肝功异常组、其他原因肝损伤组和对照组3组之间的分布频率无统计学差异(P>0.05)。结论 ABCB1基因1236C>T、2677G>T/A和3435C>T多态性与CsA所致的肝功异常无明显相关性。Objective To investigate the effects of At〉B1 genetic polymorphism on cyclosporine （CsA）-induced liver injury in Chinese renal transplant recipients. Methods ABCB1 1236C〉T, 2677G〉T/A and 3435C〉T genotypes were determined by polymerase chain reaction （PCR） followed by restriction fragment length polymorphism （RFLP） analysis in a total of 339 renal transplant recipients receiving CsA. All the subjects were divided into CsA-induced liver in- jury group, non-CsA-induced liver injury group and the control group according to the liver injury occurrence. Results In this study of 339 renal transplant recipients, the frequencies of ABCB1 1236C〉T, 2677G〉T/A and 3435C〉T mutation allele were 64. 25% ,51.54% and 35.75% respectively. The distribution frequencies of ABCB1 1236C〉T, 2677G〉T/A and 3435C〉T mutation allele had no significant difference among three groups. Conclusion There is no association be-tween ABCB1 genetic polymorphism and cyclosporine-induced liver injury in Chinese renal transplant recipients.

关 键 词：肾移植 环孢素 肝损伤 ABCB1基因 多态性 

分 类 号：R743.33[医药卫生—神经病学与精神病学]