光纤折射率传感用于壳聚糖脱乙酰度测定  

Determination of the Deacetylation Degree of Chitosans by Fiber-optic Refractive Index Sensing Technique

作　　者：何炜欣[1] 谭春华[1] 黄旭光[1] 

机构地区：[1]华南师范大学广东省微纳光子功能材料与器件重点实验室,广州510006

出　　处：《高等学校化学学报》2013年第2期474-478,共5页Chemical Journal of Chinese Universities

基　　金：国家科技支撑计划课题(批准号:2011BAF06B02);广州市科技计划项目(批准号:11C42090778)资助

摘　　要：建立了一种基于光纤折射率传感技术的壳聚糖脱乙酰度测定方法.利用光纤折射率传感器监测酸碱滴定过程中溶液折射率的变化,根据折射率变化转折点之间碱的用量来计算壳聚糖的脱乙酰度.该方法测得的3种不同含量实际样品的脱乙酰度与氢核磁共振波谱(1H NMR)方法测定结果相符,验证了方法的可靠性.该方法具有用量少、结构简单、准确、重复性好和转折点明显等优点,可应用于工业生产中壳聚糖脱乙酰度的测定.We described a novel determination method of the deacetylation degree（DD） of chitosans by fiber-optic refractive index sensing technique.The variation of the refractive index of the chitosan solution was monitored using a fiber sensor based on a two-channel Fresnel reflection technique.According to the volume of the alkali between the equivalent points,the deacetylation degree of chitosans can be calculated.With our method,DD values of three chitosan samples were determined and agreed with the results from 1H NMR spectroscopy,which proves that it is accurate and reliable for the determination of the DD of chitosans in industrial application.This method is simple,cheap to construct,with good accuracy and reproducibility.

关 键 词：壳聚糖 脱乙酰度 光纤传感 折射率 酸碱滴定 

分 类 号：O631[理学—高分子化学] O621.2[理学—化学]