芜菁不同提取物对糖尿病模型小鼠降血糖作用的研究  

Study on Hypoglycemic Effect of Different Extracts of Brassica rapa on Diabetic Model Mice

作　　者：陈湘宏[1] 文绍敦[1] 吴萍[1] 洒玉萍[1] 任世存[1] 

机构地区：[1]青海大学医学院,西宁810001

出　　处：《中国药房》2013年第7期596-598,共3页China Pharmacy

摘　　要：目的:研究芜菁不同提取物对糖尿病模型小鼠的降血糖作用。方法:给予小鼠腹腔注射链脲佐菌素复制糖尿病模型,尾静脉采血测空腹血糖,以血糖>11.1mmol/L为模型复制成功。实验分为空白对照(等容生理盐水)、模型(等容生理盐水)、二甲双胍(0.2g/kg)组和芜菁水提物高、低剂量(40、20g/kg),芜菁醇提物高、低剂量(40、20g/kg),芜菁醚提物高、低剂量(40、20g/kg)组。灌胃给药,每天1次,连续14d,实验期间分别于复制模型前、后,用药7、14d后剪尾取血检测血糖。末次给药结束后,小鼠摘眼球取血,分离血清测定超氧化物歧化酶(SOD)和丙二醛(MDA)水平,剖取新鲜的胸腺、脾脏,称质量,计算脏器指数。结果:与模型组比较,用药14d后,芜菁各提取物组小鼠血糖值显著降低(P<0.01或P<0.05);芜菁水提物高、低剂量组和芜菁醇提物高剂量组SOD活性显著增强(P<0.01或P<0.05);芜菁各提取物组MDA含量显著减少(P<0.05);除芜菁醇提物低剂量组外,各用药组胸腺指数、脾脏指数显著升高(P<0.01或P<0.05)。结论:高原植物芜菁对糖尿病模型小鼠有较明显降血糖作用。OBJECTIVE： To study the hypoglycemic effect of different extracts of Brassica rapa on diabetic model mice. METHODS： Diabetic mice model was induced by intraperitoneal injection of streptozotocin. Blood glucose monitoring devices was used to measure FPG through tail vein blood, setting blood glucose〉ll.1 mmol/L for the successful model mice. The selected mice were divided into 9 groups, i.e. blank control group （constant volume of normal saline）, model group （constant volume of normal saline）, metformin hydrochloride group（0.2 g/kg）,B, rapa water extract high-dose and low-dose groups （40,20 g/kg）, B. rapa ethanol extract high-dose and low-dose groups（40,20 g/kg） and B. rapa ether extract high-dose and low-dose groups （40, 20 g/kg）. They were given medicine intragastrically once a day for continuous 14 d. The tails blood glucose was determined before modeling, 3 d after modeling, 7 d and 14 d after treatment. After the experiment, mice eyeball blood serum was collected and sepa- rated, and SOD and MDA levels were determined. Fresh thymus and spleen were taken and weighed, and organ index was calculat- ed. RESULTS： Compared with model group, after 14 d treatment, the blood glucose level of B. rapa extract groups decreased sig- nificantly （P〈0.01 or P〈0.05） ; the activity of SOD was improved significantly in B. rapa water extract high-dose and low-dose groups and B. rapa ethanol extract high-dose group （P〈0.01 or P〈0.05） ; MDA Content decreased significantly in B. rapa extract groups; except for low-dose of B. rapa ethanol extract group, thymus index and spleen index of them increased significantly（P〈 0.01 or P〈0.05）. CONCLUSION： Highland plant B. rapa have obvious hypoglycemic effect on diabetic mice caused by strepto- zotocin.

关 键 词：高原植物 芜菁 超氧化物歧化酶 丙二醛 降血糖 链脲佐菌素 糖尿病 

分 类 号：R285[医药卫生—中药学] R96[医药卫生—中医学]