抑癌基因p16蛋白表达与外阴鳞状细胞癌关系的研究  

The Relationship between P16 expression and vulnerability and clinicopathological cheracteristics of vulvar prickle cell carcinoma

作　　者：马红梅[1] 孙聚萍[1] 

机构地区：[1]山东省青岛市市立医院妇科,青岛266071

出　　处：《中国肿瘤临床与康复》2013年第1期6-8,共3页Chinese Journal of Clinical Oncology and Rehabilitation

摘　　要：目的探讨肿瘤抑制基因p16蛋白表达与外阴鳞状细胞癌及其临床病理之间的关系。方法采用免疫组化SABC染色法检查36例外阴鳞状细胞癌组织及30例外阴良性组织中p16蛋白的表达,应用SPSS 17.0软件进行统计学处理分析。结果外阴鳞状细胞癌组织中p16蛋白的阳性表达率与外阴良性组织相比,差异有统计学意义(P<0.05)。而在不同的病理分级中,高分化组织(G1)与中低分化组织中(G2、G3)p16蛋白的阳性表达率差异有统计学意义(P<0.05)。外阴鳞状细胞癌组织中p16蛋白的表达与患者年龄、临床分期差异无统计学意义(P>0.05)。结论 p16蛋白在外阴鳞状细胞癌组织中有一定程度的表达异常,该蛋白的检测有助于判断疾病预后,指导临床治疗,并为进一步开展基因治疗提供依据。Objective To study the relationship between P16 expression and vulnerability and clinicopathological cheracteristics of vulvar prickle cell carcinoma.Methods By use of Strept Avidin-Biotin Complex,the protein of P16 was detected in 36 primary vulvar prickle cell carcinoma tissues and 30 benign vulvar tissues.Statistical analysis was performed using SPSS 17.0 for Windows.Results Low expression rate of P16 was found in vulvar prickle cell carcinoma.The protein expression deletion rate of P16 in vulvar prickle cell carcinoma was significantly higher than that in benign vulvar tissues（P0.05）.The expression level of P16 protein was higher in well-differentiated group（G1） than that of less-differentiated group（G2.G3）（P0.05）.There was no difference between the expression levels of different clinical stage and different ages.Conclusions The results suggest that loss of P16 protein expression play an important role in the generation and development of vulvar prickle cell carcinoma.Measurement of the expression of P16 protein in patients tumors might help in predicting prognosis,directing clinical treatment and providing theories for genetic therapy.

关 键 词：肿瘤抑制基因 免疫组化 P16 外阴鳞状细胞癌 

分 类 号：R737.35[医药卫生—肿瘤]