HSV1-tk基因的部分DNA序列分析  

Partial DNA Sequence Analysis of the HSV1-tk Gene

作　　者：刘彦文[1] 钟女奇[1] 都同功[1] 黄冰[1] 陈系古[1] 

机构地区：[1]中山医科大学实验动物中心,广东广州510089

出　　处：《中山大学学报（自然科学版）》2000年第3期87-90,共4页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：广东省重点攻关课题资助项目!(52 2 2 0 30 4 5);广东省博士启动基金资助项目!(984 0 58)

摘　　要：采用PCR技术从商品质粒pHSV10 6扩增出HSV1 tk基因 (112 8bp) ,PCR产物用BamHⅠ和EcoRⅠ双酶切后定向克隆至真核表达载体pcDNA3,然后以重组质粒pcTK为模板 ,tk基因的 5′端引物为DNA测序引物进行部分DNA序列分析 .部分DNA序列分析证明PCR产物为HSV1 tk基因正确无误 ,成功筛选到HSV1 tk基因的真核表达载体pcTK 。The gene coding for HSV1 tk (1 128 bp) was amplified using PCR technique. The PCR product was digested with Bam HⅠ and Eco RⅠ and cloned into the plasmid pcDNA3 at the Bam HⅠ and Eco RⅠ sites. The recombinant plasmid pctk was identified by restriction analysis and partial DNA sequence analysis. The results show that HSV1 tk gene was successfully amplified and was correctly cloned into the Bam HⅠ and Eco RⅠ site of plasmid pcDNA3.

关 键 词：HSV1-TK 基因治疗 转基因动物 克隆 DNA序列分析 

分 类 号：R394[医药卫生—医学遗传学]