乳腺癌高表达基因FAM83A的生物信息学分析  

Bioinformatics analysis of the highly expressed FAM83A gene in breast cancer

作　　者：刘镭[1] 黄亮[2] 车清海 赵恩宏[2] 黄旭[2] 程露阳[1] 肖丽君[1] 

机构地区：[1]承德医学院基础医学部免疫教研室,河北承德067000 [2]承德医学院附属医院肿瘤诊治中心,河北承德067000 [3]吉林市龙潭区妇幼保健院妇产科,132000

出　　处：《重庆医学》2013年第3期243-245,共3页Chongqing medicine

基　　金：河北省人口与计划生育委员会资助项目(2009-B21);河北省卫生厅资助项目(20090579)

摘　　要：目的筛选乳腺癌高表达基因,用生物信息学方法预测FAM83A生物学功能。方法使用肿瘤基因组解剖计划数据库的数字基因表达演示工具,在乳腺癌组织和乳腺良性肿瘤组织中筛选差异表达的基因。生物信息学方法预测FAM83A基因及其编码蛋白的序列同源性、理化性质、亚细胞定位、结构和表达谱。RT-PCR方法测定FAM83A在乳腺癌、乳腺纤维瘤和正常乳腺组织中的表达。结果筛选出差异表达基因FAM83A。发现其有3个不同的剪接体,基因进化保守,亚细胞定位于线粒体的可能性大。预测有PK,PKA等磷酸化位点,MAPK作用识别位点,SH2、SH3,RPEL和WH2结合基序。二级结构主要是α螺旋和无规则卷曲。RT-PCR证实FAM83A在乳腺癌组织中高表达,而在乳腺良性肿瘤和正常乳腺组织中均无表达。结论 FAM83A是一个乳腺癌高表达基因,可能作为乳腺癌分子机制研究的靶点。Objective To screen and find over-expressed gene in breast cancer,and to predict the function of FAM83A by bioinformatics.Methods We used digital gene expression displayer（DGED） tool in cancer genome anatomy project（CGAP） to screen the differentially expressed genes between the breast cancer and benign tumor.The sequence homology,properties,subcellular location,structure and expression profile of FAM83A were analyzed by bioinformatics software.At last,we detected the expression of FAM83A in breast cancer,breast fibroadenoma and normal breast tissue by RT-PCR.Results We found a differentially expressed gene FAM83A which had three transcript variants.It was evolutionary conservation.Bioinformatics analysis indicated that FAM83A protein was located likely in the mitochondria.There were PK,PKA phosphorylation sites,MAPK recognition site,SH2,SH3,RPEL and WH2 binding motif.The secondary structure mainly comprised of alpha helices and random coils.RT-PCR results confirmed that FAM83A was over-expressed in breast cancer tissues,while no expression in benign breast tumor and normal breast tissue.Conclusion FAM83A is over-expressed in breast cancer and maybe act as a study target for molecular mechanism.

关 键 词：乳腺肿瘤 生物信息学 SAGE库 FAM83A 

分 类 号：R737.9[医药卫生—肿瘤]