2-脱氧葡萄糖联合顺铂对胰腺癌细胞系的体外抑制作用  

The effects of glycolysis inhibitor 2-Deoxyglucose combining with cisplatin against different types of pancreatic cancer cells

作　　者：穆喆[1] 耿希刚[1] 

机构地区：[1]西安交通大学医学院第一附属医院,西安710061

出　　处：《陕西医学杂志》2013年第2期144-146,共3页Shaanxi Medical Journal

摘　　要：目的:探讨糖酵解抑制剂2-脱氧葡萄糖(2-DG)联合广谱抗肿瘤药物顺铂对不同种系胰腺癌细胞系在不同情况下的体外抑制作用以及其机制。方法:使用2-DG以及联合顺铂对2种胰腺癌细胞系(Mia,Panc-1)实施生长干预,进行2-DG对不同细胞系抑制作用的MTT试验,及对细胞系进行凋亡的相关检测。结果:2-DG对不同种系的胰腺癌细胞抑制以及凋亡作用效果不同,但都呈时间反应性增加;2-DG联合顺铂较单药作用对细胞的抑制作用更为明显。结论:不同种系胰腺癌细胞系对2-DG都有一定的时间依赖性抑制作用,机制可能为2-DG对细胞凋亡的促进作用,2-DG在体外实验中体现一定的抗肿瘤活性以及联合化疗的活性,可能在胰腺癌的辅助化疗以及新辅助化疗等治疗中起到一定的作用。Objective：To discuss the effects of glycolysis inhibitor 2-Deoxyglucose combining with cispla- tin against different types of pancreatic cancer cells, and to detect the preliminary mechanism about it. Methods：Cells （MiaPaCa2 ＆ pane-1 ）incubated with lmg/L 2-Deoxyglucose and cisplatin. A apoptosis and Caspase-3 detected were done in 8 hours. After 24 and 48 hours, ceils were under MTT detect. Results： Having positive inhibit effect on pancreatic cancer cells, 2-Deoxyglucose＇s anti-cancer effect has an time-depend effect, and combining with cisplatin, it rised its effect on cancer cells. Conclusion： 2 types of pancreatic cancer cells explained inhibited by 2 Deoxyglucose. Probably the mechanism lies in the apoptosis induces through caspase-3, and that may has a certain effect on anti- cancer chemotherapy or neoadjuvant chemotherapy in the next period.

关 键 词：胰腺肿瘤 药物疗法 脱氧葡萄糖 治疗应用 顺铂 治疗应用 分子药理作用机制细胞凋亡 

分 类 号：R735.9[医药卫生—肿瘤]