光动力作用对人结肠癌细胞转录因子激活蛋白-4表达的影响  

Inhibition of AP-4 gene expression by zinc phthalocyanine-mediated photodynamic therapy in human colon cancer cell line SW480

作　　者：张通[1] 曹杰[1] 杨平[1] 李旺林[1] 孙政[1] 张伟健[1] 曾山崎[1] 

机构地区：[1]广东省广州市第一人民医院胃肠外科,510180

出　　处：《广东医学》2013年第2期173-176,共4页Guangdong Medical Journal

基　　金：广东省科技计划项目(编号:2010B060900016);广州市医药卫生科技重点项目(编号:2009-ZDi-01)

摘　　要：目的观察不同浓度光敏剂酞菁锌对结肠癌细胞株SW480的生长抑制作用及其对转录因子激活蛋白-4(AP-4)表达的影响。方法应用CCK-8方法评估光动力作用后SW480细胞的存活率,通过流式细胞技术、RT-PCR技术、Western blot技术检测光敏剂酞菁锌光动力作用后对SW480细胞凋亡和AP-4基因表达等生物学行为的影响。结果酞菁锌光动力治疗对结肠癌细胞株SW480生长增殖具有明显抑制作用,其效应呈浓度和光照剂量依赖性;流式细胞仪分析显示SW480细胞呈G2/M期阻滞;SW480细胞的凋亡率随酞菁锌浓度增加逐步上升。2.0μg/mL酞菁锌光动力作用SW480细胞48 h后其AP-4 mRNA水平下降了81%,培养液上清液AP-4蛋白浓度下降了75.6%(P<0.01)。结论应用光敏剂酞菁锌光动力作用能有效抑制SW-480细胞AP-4的表达,进而抑制细胞的生长、增殖及诱导细胞的凋亡,为结肠癌治疗提供了新的思路和手段。Objective To investigate the influence of zinc phthalocyanine on the proliferation and AP -4 gene expression in SW480 cells in vitro. Methods The cell proliferation was analyzed by cell counting kit - 8 （CCK8） assay, while the AP- 4 mRNA and protein expression after photodynamic therapy （PDT） were analyzed by RT- PCR and West- ern blot, respectively. Meanwhile, the flow cytometry （FCM） was used to detect the cell apoptosis and cycle. Results The proliferation of SW480 cells was significantly suppressed by zinc phthalocyanine - mediated PDT in concentration - and light dose- dependent manners. The cell was arrested at G2/M by PDT, while the apoptosis rate was enhanced with zinc phthalocyanine in concentration dependent manner. After 2.0 p,g/mL zinc phthalocyanine - mediated PDT, the AP - 4 mRNA and protein were significantly reduced by 81% and 75.6% , respectively, in SW480 eells （P 〈0. 01 ）. Conclu- sion Zinc phthalocyanine - mediated PDT can effectively suppress the expression of AP - 4, and inhibit the cell growth and proliferation, and induce apoptosis in SW480 cells, suggesting novel treatment of human colon cancer.

关 键 词：结肠癌 激活蛋白-4 酞菁锌 光动力 

分 类 号：R575.02[医药卫生—消化系统]