亚甲基四氢叶酸还原酶C667T基因多态性对以5-氟尿嘧啶为基础化疗的晚期胃癌患者生存期的影响  

Influence of methylene tetrahydrofolate reductase C667T gene polymorphism on survival time ofadanced gastric cancer patients treated with 5-fluorouracil-based chemotherapy

作　　者：季枚[1] 蒋敬庭[1] 徐斌[1] 吴骏[1] 赵伟庆[1] 吴昌平[1] 

机构地区：[1]苏州大学附属第三医院肿瘤中心,常州213003

出　　处：《中华实验外科杂志》2013年第2期242-244,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（81171653、30972703）;江苏省自然科学基金资助项目（BK2011246、BK2011247）;六大人才高峰第六批资金资助项目（BRA2010037）;江苏省卫生厅医学科技发展基金资助项目（P200932、P200933）;常州市社会发展计划基金资助项目（CE2012501F、CS20092019、CS20102020）;常州市卫生局重大招标项目（ZD200810）

摘　　要：目的观察亚甲基四氢叶酸还原酶（MTHFR）C667T基因多态性与5一氟尿嘧啶（5．Fu）为基础的化疗方案治疗晚期胃癌的疗效。方法收集经病理学确诊的晚期胃癌59例。所有病例化疗前抽取外周静脉血，提取DNA，用连接酶检测反应技术（LDR）检测研究对象的MTHFR基因型。患者均采用经5一Fu为基础的联合化疗方案。结果59例晚期胃癌患者MTHFRrr／T基因型患者的无进展生存期（PFS）明显优于C／C型（8．70个月比5．47个月,X2：9．209，P〈0．01），MTHFRrr／T基因型患者的PFS明显优于C／T型（8．70个月比5．08个月，∥=9．405，P〈0．01），MTHFRC／T基因型患者的PFS与C／C型比较差异无统计学意义（5．47个月比5．08个月,X2=0．092，P〉0．05）。结论MTHFR基因型对预测以5．Fu为基础化疗方案治疗晚期胃癌的疗效有较好的临床意义。Objective To study the methylene tetrahydrofolate reductase （MTHFR） C667T gene polymorphism and the therapeutic effect of 5-fluorouraeil （5-Fu）-based chemotherapy for adaneed gastric cancer patients. Methods Fifty-nine cases of pathologically diagnosed adanced gastric cancer were collect- ed. Peripheral venous blood of all patients was drawn before chemotherapy, and DNA was extracted, and then the MTHFR genotype was detected with ligase detection reaction （LDR）. All patients were treated with 5-Fu-based combined chemotherapy. Results Progression-free survival （PFS） of adanced gastric cancer patients with MTHFR T/T genotype was significantly longer than that of patients with MTHFR C/C genotype （ 8.70 months vs. 5.47 months ,X2 = 9. 209, P 〈 0. 01 ）, and that of patients with MTHFR TIT genotype was significantly longer than that of patients with MTHFR C/T genotype （ 8. 70 months vs. 5.08 months,）（2 =9. 405 ,P 〈0. O1 ）. There was no significant difference in PFS between patients with MTHFR C/T genotype and those with MTHFR C/C genotype （5.47 months vs. 5.08 months,x2 = 0. 092, P 〉 0. 05 ）. Conclusion MTHFR genotype has good clinical significance for predicting the therapeutic effect of 5-Fu-based chemotheralav for adanced ~astric cancer.

关 键 词：胃癌 亚甲基四氢叶酸还原酶 基因多态性 化学治疗 

分 类 号：R735.2[医药卫生—肿瘤]