红色／绿色荧光蛋白示踪胶质瘤干祖细胞在肿瘤血管形成中的作用  

The effect of glionm stem/progenitor cell on tumor angiogenesis traced by red/green fluorescentprotein

作　　者：朱玉德[1] 代兴亮[1] 赵东亮[1] 孙超[1] 董军[1] 黄强[1] 

机构地区：[1]苏州大学附属第二医院神经外科,215004

出　　处：《中华实验外科杂志》2013年第2期297-299,共3页Chinese Journal of Experimental Surgery

基　　金：国家重点基础研究发展计划（973资助项目）（2010CB5.29400）;国家自然科学基金资助项目（81172400、81071766、81272799）

摘　　要：目的探讨胶质瘤干祖细胞在胶质瘤血管形成过程中所起的作用。方法将转染红色荧光蛋白（RFP）基因的胶质瘤干祖细胞，原位（25ul，1×10。个细胞）或皮下（200p,1，1x10。个细胞）植入4—6周龄的表达绿色荧光蛋白（GFP）的裸小鼠内，2～3周致瘤后取移植瘤组织压成簿片后在荧光、激光共聚焦显微镜下观察肿瘤血管。结果在22个标本中根据颜色区分出血管的组成细胞有宿主源性（绿色）、肿瘤源性（红色）和两者的融合细胞（黄色）；组成肿瘤血管的细胞类型有：内皮依赖（EC），遍布肿瘤组织内；肿瘤细胞依赖型（TC）的血管拟态（VM）和EC／TC嵌合型（马赛克血管，MC）。其中VM、MC多位于肿瘤组织中心，肿瘤边缘未见到。结论携RFP的胶质瘤干祖细胞移植入表达GFP的裸小鼠体内的移植瘤模型是研究肿瘤血管发生的良好模型；胶质瘤干／祖细胞可能是肿瘤血管形成的启动细胞。Objective To explore the potential role of glioma stem cell in the generation and ma- intenance of tumor blood vessels. Methods Gliomas stem cells （ CD133 ＋ tumor cells） transfected with red fluorescent protein （RFP） gene were implanted in situ stereotaxicly （25 ~1,1 x 106 cells） or subcuta- neously （200 p,1,1 x 10s cells） in nude mice, then the xenografts （2-3 weeks） were pressed flat and tumor vessels structures were observed under fluorescence microscope and laser confocal microscope. Results Tissue squash method can clearly display the host normal vessels and some cells source of vascular endothe- lial cells inside the tumor region on the basis of different colors under fluorescence, laser confocal micro- scope. Images displayed vascular cells can be classified colors into three types ： host derived cells （ green）, tumor derived（red） ,tumor derived and host-derived fusion（yellow）. Cells form of tumor angiogenesis can be divided into three types：proliferation of endothelial cells（ EC）, tumor stem cells （TC）, tumor stem cells and host-derived endothelial cells fusion （EC/TC）. Conclusion Gliomas stem cells transfected with red fluorescent protein （RFP） gene were implanted in green fluorescent protein （GFP） transgenic nude mice model was very useful to study the processes of malignant glioma angiogenesis. This model can prove the gli- oma stem/progenitor cells act as initiating cell in tumor angiogenesis.

关 键 词：肿瘤干细胞 荧光蛋白基因转染 荧光裸小鼠 可移植性肿瘤 肿瘤血管生成 

分 类 号：R730.2[医药卫生—肿瘤]