分化抑制因子1、3及基质金属蛋白酶9在脑转移瘤组织中的表达及意义  

Expression and significance of differentiation inhibitor 1,3 and matrix metalloproteinase-9 in brainmetastatic tumors

作　　者：李建峰[1] 扈玉华[2] 董雅兰[1] 

机构地区：[1]河北医科大学第四医院神经外科,石家庄050000 [2]河北医科大学第二医院神经外科,石家庄050000

出　　处：《中华实验外科杂志》2013年第2期300-302,共3页Chinese Journal of Experimental Surgery

摘　　要：目的观察分化抑制因子1（Id-1）、Id-3和基质金属蛋白酶9（MMP-9）mRNA、蛋白在脑转移瘤组织中的表达，探讨它们在脑转移瘤形成和发展过程中的作用及机制。方法对39例脑转移瘤标本采用逆转录一聚合酶链反应（RT—PCR）和免疫组织化学法，观察Id-1、Id-3、MMP-9mRNA及蛋白表达，并探讨它们表达之间的相关性。结果Id-1、Id-3、MMP-9mRNA在脑转移瘤中的相对表达量分别为0．75±0．07、0．83±0．06、0．72±0．09，明显高于正常脑组织（P〈0．05）；三者蛋白在脑转移瘤中的阳性表达率分别为92．3％、94．9％、79．5％，与正常脑组织比较表达明显增高（P〈0．05）。Id-1、Id-3与MMP-9mRNA及蛋白表达均呈显著正相关（r=0．569，P〈0．01；r=0．386，P〈0．05）。结论Id-1、Id一3、MMP-9在脑转移瘤的形成与发展中发挥重要作用，且三者存在一定的协同作用。Objective To observe the expression of differentiation inhibitor 1 （Id-1）, Id-3 and matrix metalloproteinase-9 （MMP-9） in cerebral metastatic tumors and to explore their roles and mecha- nism in the formation and development of cerebral metastases. Methods The expression of Id-1, Id-3 and MMP-9 mRNA and protein in cerebral metastases was detected by using reverse transcription-polymerase chain reaction （RT-PCR） and immunohistochemistry. The correlation of their expression was assayed sta- tistically. Results The relative expression levels of Id-1, Id-3 and MMP-9 mRNA were 0. 75 ＋ 0. 07, 0. 83± 0. 06 and 0. 72 ± 0. 09 respectively in brain metastases, obviously higher than those in human nor- mal brain tissues （P 〈0. 05）. The positive expression rate of Id-1, Id-3 and MMP-9 protein was 92. 3% , 94. 9% and 79.5% respectively in brain metastases, obviously higher than those in in human normal brain tissues （ P 〈 0. 05 ）. The expression of Id-1, Id-3 mRNA and protein had a positive correlation with that of MMP-9 in metastases （ r = 0. 569, P 〈 0. 01 ; r = 0. 386, P 〈 0. 05 ）. Conclusion The over-expression of Id-1 , Id-3 and MMP-9 played an important biological function in the formation of brain metastatic tumor, and there was a synergistic effect among them to some extent.

关 键 词：分化抑制因子1 3 基质金属蛋白酶9 脑转移瘤 

分 类 号：R73[医药卫生—肿瘤]