超声心动图对阿霉素心肌病模型大鼠左心收缩功能的评价  

Evaluation of left ventricular function in rat adriamycin-induced dilated cardiomyopathy model byusing echocardiography

作　　者：郭文清[1] 赵子牛[2] 袁建军[1] 刘洪智[3] 

机构地区：[1]河南省人民医院超声科,郑州450000 [2]河南省人民医院超声心外科,郑州450000 [3]河南省人民医院心内科,郑州450000

出　　处：《中华实验外科杂志》2013年第2期317-318,共2页Chinese Journal of Experimental Surgery

摘　　要：目的探讨超卢心动冈技术在评估阿霉素心肌病模型大鼠心功能的优势及应州价值。方法将硝只雄性Wistar大鼠按体质量随机分2组：一组制备阿霉素心肌病模型。正常对照组注射等量生理盐水。丁实验第12周应用VIVlD7彩色超声显像仪行二维、多普勒及M型超声检测评价其左心收缩功能，并作苏小素一伊红（HE）染色观察心肌组织学变化。结果（1）死亡率：阿霉素心肌病组大鼠12周累计死亡10只，死亡率为40％。，死亡原凶为充肌性心力衰竭；正常对照组无死亡。（2）大鼠心脏超声测定结果：阿霉素心肌病组大鼠左室收缩末期内径[（0．44±0．06）cm]及舒张末期内径[（0．67±0．07）cm]大于正常对照组（P〈0．01），左室短轴缩短率[（33．94-4-3．56）％]低于正常对照组（P〈0．01），主动脉射血速度[（0．71±0．10）m／s]低于正常对照组（P〈0．01），病理学结果证实阿霉素心肌病组大鼠心肌符合心肌病样改变。结论阿霉素常规剂量多次静脉注射，可致大鼠发生心脏问质纤维化及心脏收缩功能降低。，超声心动图技术能够敏感、快速、尢创性地评价阿霉素心肌病大鼠左心室收缩功能的变化。Objective To evaluate the left ventricular （LV） function in the rat adriamycin-in- duced dilated eardiomyopathy model （ADR-DCM） . Methods Thirty-five adult male Wistar rats were ran- domly divided into 2 groups. The ADR-DCM group （ n = 25） was injected with a（iriamyein at a dose of 2.5 mg/kg intravenously once a week tbr l0 weeks, and the control group （n = 10） received an equivalent volume of 0. 9% saline alone intravenously. Echoeardiographie measurements were obtained at 12th week after treatment. The pathological changes of LV were examined by hematoxylin-eosin staining. Results The cumulative mortality in ADR-DCM group was 40%. As compared with control group, LV end-diastolic diameter [ LVEDD, （0. 67 ±0. 07） cml, LV end systolic diameter [ LVESD, （0. 44 ±0. 06） cml, and the hydroxyproline and collagen eontents were significantly increased （ all P 〈 0. 01 ） , short-axis fractional shortening （FS, 33.94±3.56）% and the systolic blood flow velocity of aorta （0. 71± 0. 10） m/s were significantly reduced （ all P 〈 0. 01 ） in ADR-DCM group. The pathological changes in ADR-DCM group were consistent with those of cardiomypathy. Conclusion （ 1 ） Chronic administration of adriamycin at a regular dose intravenously to rats could result in left ventricular myocardial fibrosis and heart falure; （2） Echocardiography can sensilively, rapidly and non-invasively evaluate the left ventricular function of the rat ADR-DCM.

关 键 词：阿霉素 心肌病 超声心动图技术 

分 类 号：R-332[医药卫生] R542