纯钛表面纳米HA涂层的制备及生物学评价  

Preparation and biological evaluation of Nano-HA coating on tatinium surfaces

作　　者：董玲玲[1] 杨国利[1] 方文[1] 赵士芳[1] 何福明[1] 陈晖[1] 

机构地区：[1]浙江大学医学院附属口腔医院,杭州310006

出　　处：《现代口腔医学杂志》2013年第1期15-19,共5页Journal of Modern Stomatology

基　　金：浙江省科技厅公益性项目(2011C33034)

摘　　要：目的采用电化学法在粗化钛片表面制备纳米HA(Nano-HA)涂层并进行生物学评价。方法钛片表面粗化,采用电化学法在粗化钛片表面沉积Nano-HA涂层。通过场发射扫描电子显微镜(FSEM)、傅立叶红外光谱(FTIR)和X射线衍射仪(XRD)观察涂层晶体形貌、化学结构及组成;通过大鼠骨髓基质干(BMSCs)细胞培养验证该涂层的生物活性。结果 FSEM证实Nano-HA沉积在粗化钛片表面,直径约70～80nm,棒状晶粒截面为规则的六变形。FTIR和XRD分析表明该涂层为HA。与对照组相比,Nano-HA能够明显促进碱性磷酸梅(ALP)和骨钙素(OC)的表达。结论采用电化学法可在粗化纯钛表面制备Nano-HA涂层,并可促进BMSCs成骨分化。Objective This study applied an electrochemical technology to prepare a nano-hydroxyapatite（nano-HA） coating and evaluated the effects of the nano-HA coating on osteoblast biological ability.Methods Titanium plates were roughened.Nano-HA coatings were deposited by the electrochemical technology.FSEM,FTIR and XRD analysis were used to observe topography and chemical composition of coatings.The biological activity was evaluated by the BMSCs cell culture.Results FSEM observation showed the rod-like crystals uniformity covered the porous surfaces,the diameter of which was 70～80nm.XRD and FTIR analysis showed this coating consisted of HA.Nano-HA coatings improved expression of ALP and OC.Conclusion Nano-HA coatings were deposited on rough titanium surfaces by the electrochemical technology and improved BMSCs into osteoblast.

关 键 词：Nano-HA 电化学法 BMSCS 钛 

分 类 号：R783.1[医药卫生—口腔医学]