人结直肠癌化疗药物的裸鼠体内敏感性实验  

Sensitivity Test of Chemotherapy on Human Colorectal Cancer in Vivo

作　　者：甄运寰[1] 颜登国[1] 张东兴[2] 李国胜[1] 龚慧[1] 

机构地区：[1]贵阳医学院附属医院肛肠外科,贵州贵阳550004 [2]贵州省人民医院普通外科,贵州贵阳550001

出　　处：《中国普外基础与临床杂志》2013年第1期48-53,共6页Chinese Journal of Bases and Clinics In General Surgery

基　　金：贵州省优秀科技人才省长专项基金(项目编号:黔省专合字2005-61);贵阳市科技局社会发展基金(项目编号:T2004-13)~~

摘　　要：目的探讨活体内人结直肠癌对5种化疗药物的敏感性。方法用9例结直肠癌患者的肿瘤组织建立结直肠癌裸鼠皮下移植瘤模型,分别应用5-氟尿嘧啶(5-FU)、奥沙利铂(LOHP)、丝裂霉素(MMC)、阿霉素(ADM)及伊立替康(CPT-11)对移植瘤进行干预治疗,观察该5种药物对裸鼠皮下移植瘤生长的影响。结果对照组裸鼠皮下移植瘤的体积明显大于其余5个化疗药物组;6组裸鼠肝肺均无转移。5种化疗药物对9例患者的裸鼠皮下移植瘤的最低和最高抑瘤率5-FU为23.6%和54.9%;LOHP为23.7%和69.5%;CPT-11为23.6%和82.6%;MMC为24.1%和48.1%;ADM为5.8%和20.7%。LOHP、CPT-11、5-FU和MMC组分别有7例、6例、4例和1例患者的裸鼠皮下移植瘤抑瘤率在40.0%以上。9例患者的移植瘤中,3种药物抑瘤率均在40.0%以上者3例,2种药物抑瘤率均在40.0%以上者4例,1例除CPT-11的抑瘤率为82.6%外,其余4种药物的抑瘤率均在30.0%以下,1例5种化疗药物的抑瘤率均在31.0%以下。5个化疗药物组抑瘤率之间的差异具有统计学意义(H=24.061 2,P=0.000 1),其中,与ADM组相比,5-FU组、MMC组、LOHP组及CPT-11组的抑瘤率较高(P<0.05),后4组之间抑瘤率的差异均无统计学意义(P>0.05)。结论同一结直肠癌患者的裸鼠皮下移植瘤对不同化疗药物的敏感性存在较大差异,同一种化疗药物对不同结直肠癌患者的裸鼠皮下移植瘤的抑瘤率也存在较大差异。在5种化疗药物中,LOHP和CPT-11对裸鼠皮下结直肠癌移植瘤的抑瘤率最高,其次为5-FU和MMC。Objective To investigate the sensitivity of 5 kinds of chemotherapeutic drugs on human colorectal cancer in vivo.Methods Xenografts in nude mice were set up by tumor tissues from 9 patients with colorectal cancer and nude mice were divided into 6 groups randomly,receiving saline（control group）,5-fluorouracil（5-FU group）,doxorubicin（ADM group）,mitomycin（MMC group）,oxaliplatin（LOHP group）,and irinotecan（CPT-11 group）,respectively.The inhibitive rates（IR） of xenografts in 5 groups for each patient were calculated.Results The lowest and highest IR of 5 groups were 23.6% and 54.9% in 5-FU group,23.7% and 69.5% in LOPH group,23.6% and 82.6% in CPT-11 group,24.1% and 48.1% in MMC group,5.8% and 20.7% in ADM group,respectively.The IR exceeded 40.0% in 7 patients of LOHP group,6 patients of CPT-11 group,4 patients of 5-FU group,and 1 patient of MMC group,respectively.Of 9 patients,the IR exceeded 40.0% to 3 kinds of drugs in 3 patients,to 2 kinds of drugs in 4 patients,the IR didn＇t exceed 30.0% to 4 kinds of drug（IR was 82.6% to CPT-11） in 1 patient,and the IR didn＇t exceed 31.0% to all 5 kinds of drugs in 1 patient.There were statistical differences on the IR of 5 kinds of drugs（H=24.061 2,P=0.000 1）.IR of ADM group was statistical lower than 5-FU group,MMC group,LOHP group,and CPT-11 group（P0.05）,but there were no statistical differences between 5-FU group,MMC group,LOHP group,and CPT-11 group（P0.05）.Conclusions The xenografts from same patient have different sensitivity to different chemotherapy drugs,and the same chemotherapy drug corresponds to different IR in different patients.The IR of LOHP and CPT-11 are the highest,following by 5-FU and MMC.

关 键 词：结直肠癌 化疗 药物敏感性 体内实验 

分 类 号：R735.3[医药卫生—肿瘤] R73-361[医药卫生—临床医学]