遗传性非息肉病性结直肠癌hMLH1和hMSH2蛋白表达与其临床病理特征及预后的关系  

Relationship Between The Expressions of hMLH1 and hMSH2 Protein and Clinicopathological Features and Prognosis of Hereditary Nonpolyposis Colorectal Cancer

作　　者：孟金[1] 李晓霞[1] 赵萌[1] 唐元新[1] 孙公平[1] 李鑫 闫一飞[1] 

机构地区：[1]中国医科大学附属第四医院胃肠外科,辽宁沈阳110032

出　　处：《中国普外基础与临床杂志》2013年第1期66-70,共5页Chinese Journal of Bases and Clinics In General Surgery

基　　金：辽宁省教育厅高等学校科研项目(项目编号:2008781);2011年辽宁省自然科学基金项目(项目编号:201102281)~~

摘　　要：目的探讨错配修复(MMR)基因hMLH1和hMSH2的蛋白表达与遗传性非息肉病性结直肠癌(HNPCC)临床病理特征及预后的关系。方法应用免疫组化方法(Elivision-两步法)检测48例符合修订后的《Bethesda指南》的HNPCC患者中hMLH1和hMSH2蛋白的表达情况,并定义两者同时表达阳性为MMR蛋白表达,分析其表达与HNPCC临床病理特征及预后的关系。结果 hMLH1蛋白的表达缺失率为20.83(10/48),高于hMSH2蛋白的表达缺失率(8.33%,4/48),Ρ<0.05;MMR蛋白的表达阳性率为70.83%(34/48)。MMR蛋白表达与肿瘤的浸润深度相关(Ρ<0.05)。MMR蛋白表达缺失与表达正常者的总生存率分别为85.71%(12/14)和85.29%(29/34),两者生存曲线比较差异无统计学意义(Ρ>0.05)。结论 hMLH1蛋白的表达缺失率高于hMSH2蛋白,hMLH1和hMSH2蛋白表达缺失与肿瘤的浸润深度相关,与患者的预后无关。Objective To investigate the relationship between the expressions of mismatch repair（MMR） genes（include hMLH1 and hMSH2） and clinicopathological features and prognosis of hereditary nonpolyposis colorectal cancer（HNPCC）.Methods Immunohistochemistry method（Elivision-two step） was used to test expressions of hMLH1 and hMSH2 proteins（both hMLH1 and hMSH2 protein-positive delimited as MMR protein-positive） in 48 patients with HNPCC according to revised Bethesda guidelines,and analyzed the relationship between the expression of MMR protein and clinicopathological features and prognosis of HNPCC.Results Loss rate of hMLH1 protein（20.83%,10/48） was significantly higher than that of hMSH2 protein（8.33%,4/48）,Ρ0.05,and positive expression rate of MMR protein was 70.83%（34/48）.Expression of MMR protein was related with tumor infiltration depth（Ρ0.05）.Survival rate of patients with expression and without expression of MMR protein was 85.29%（29/34） and 85.71%（12/14）,respectively,the survival curves of them didn＇t significantly differed from each other（Ρ0.05）.Conclusions Loss rate of hMLH1 protein is higher than that of hMSH2 protein.Expre ssions of hMLH1 and hMSH2 protein are related with tumor infiltration depth,but not related with prognosis.

关 键 词：遗传性非息肉病性结直肠癌 错配修复基因 免疫组织化学 

分 类 号：R735.35[医药卫生—肿瘤] R735.37[医药卫生—临床医学]