细胞色素P450 2E1在依达拉奉减轻免疫性肝损伤氧化应激中的作用  

Role of cytochrome P450 2E1 in edaravone easing oxidative stress in immune liver injury

作　　者：陈方军[1] 李俊[2] 

机构地区：[1]安庆医药高等专科学校临床医学系,安徽安庆246052 [2]安徽医科大学药学院,安徽合肥230032

出　　处：《中国药理学通报》2013年第2期216-220,共5页Chinese Pharmacological Bulletin

基　　金：安徽省教育厅自然科学研究项目(No KJ2009B209Z)

摘　　要：目的观察细胞色素P450 2E1(CYP 2E1)在依达拉奉作用于刀豆蛋白A(Con A)致小鼠免疫性肝损伤模型中的变化。方法先期给予依达拉奉,之后用Con A腹腔注射致敏小鼠产生急性免疫性肝损伤,观察肝脏病理变化,分光光度法测血清AST和ALT浓度,肝匀浆中SOD、MDA、GSH含量,RT-PCR技术检测肝脏组织CYP 2E1 mRNA水平,Westernblot技术检测肝脏组织CYP 2E1蛋白表达水平。结果与模型组比较,依达拉奉能降低免疫性肝损伤小鼠血清中升高的ALT、AST含量;降低肝匀浆中的MDA水平,升高其降低的SOD、GSH水平;CYP 2E1 mRNA水平明显降低,CYP 2E1蛋白表达水平明显下调。结论依达拉奉对小鼠急性免疫性肝损伤具有一定的保护作用,这可能与它降低CYP 2E1的表达,清除自由基,增强机体抗脂质过氧化能力有关。Aim To observe the effect of edaravone on the cytochrome P450 2E1（CYP 2E1） in immune liver injury model of mice induced by concanavalin A（Con A） Methods After edaravone was injected,the mice were sensitized by Con A via intraperitoneal injection and acute immune liver damage was produced.The liver pathological changes were observed,serum ALT,AST concentration,the levels of MDA,SOD and GSH in liver were measured by spectrophotometry.Expression of CYP 2E1 in liver was detected by reverse transcription-polymerase chain reaction（RT-PCR） assay and Western blot.Results Compared the model group,edaravone could reduce serum ALT,AST concentration;it also reduced MDA level,and raised SOD,GSH activities in liver homogenate.Edaravone treatment significantly decreased CYP2E1 mRNA level and CYP 2E1 protein expression in mouse liver tissues.Conclusion In acute immune liver injury of mice induced by Con A,edaravone has certain protective effect,which it may be related with reducing CYP 2E1 expression,eliminating free radicals and lipid peroxidation,and enhancing the antioxidant capacity.

关 键 词：依达拉奉 氧化应激 自由基 肝损伤 CONA 细胞色素P450 2E1 

分 类 号：R-332[医药卫生] R322.47