女贞子治疗去卵巢大鼠骨质疏松的实验研究  

Treatment with FLL for osteoporosis in ovariectomized rats

作　　者：程敏[1] 王庆伟[2] 刘雪英[2] 邓雅婷[2] 贾朝[2] 

机构地区：[1]商洛学院生物医药工程系,陕西商洛726000 [2]第四军医大学,陕西西安710032

出　　处：《中国药理学通报》2013年第2期229-233,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 30973907);陕西省教育厅自然科学基金资助项目(No 12JK1009)

摘　　要：目的观察女贞子对去卵巢(ovariectomy,OVX)大鼠骨质疏松(osteoporosis,OP)的治疗作用,并探讨其作用机制。方法大鼠骨质疏松模型,女贞子(9、4.5、2.25 g.kg-1.d-1)灌胃给药,实验过程称体重,连续给药26周后测定血钙(S-Ca)、血磷(S-P)、尿钙(U-Ca)、尿磷(U-P)的含量,ELISA试剂盒双抗体夹心法测定血清碱性磷酸酶(ALP)、血清骨特异性碱性磷酸酶(BALP)含量、骨钙素(OCN)含量、尿液中脱氧吡啶啉(DPD)含量,DEXA型骨密度仪分析大鼠的股骨、胫骨、第4椎骨的BMD(bone mineral density),动物处死后完整取下心、肝、脾、肺、肾、胸腺、脑、子宫进行称重,采用HE染色法进行子宫病理学检查。结果女贞子能有效抑制去卵巢所致的大鼠体重增加、升高S-Ca、S-P、降低U-Ca、U-P、降低ALP、BALP、OCN、DPD的含量,增加大鼠股骨、胫骨、第4椎骨的BMD,并且长期用药对子宫无明显刺激作用。结论女贞子对OVX大鼠所致的骨质疏松症有良好的治疗效果,其作用机制可能与其能降低高的骨转换率有关。Aim To systematically evaluate the therapeutic effect of Fructus Ligustri Lucidi（FLL）on ovariectomy（OVX） induced osteoporosis in rats,and to explore the mechanism of the antiosteoporotic effects of FLL on osteoporosis.Methods Daily oral administration of FLL（9、4.5、2.25 g·kg^-1·day^-1,n=10） started from 5th week for 26 weeks after OVX.The body weight of the animals was recorded weekly during the experimental period.Bone turnover markers（Serum alka-line phosphatase（ALP）,bone-specific alkaline phosphatase（BALP）,osteocalcin（OCN）,deoxypyridinoline（DPD） and other parameters including serum calcium（S-Ca）,serum phosphorus（S-P）,urine calcium（U-Ca）,phosphorus（U-P）,and bone mineral density（BMD） of the femur,4th lumbar vertebra and tibia were measured.Heart,liver,spleen,lung,kidney,thymus,brain and uterine were removed from each rat and immediately weighed.HE staining method was used for uterine pathological examination.Results Administration of FLL over a 26-week period significantly prevented ovariectomy-induced bone loss and the body weight gain without affecting the weight of the uterus,and increased S-Ca,S-P levels,decreased level of bone turnover markers and U-Ca,U-P,ALP,BALP,OCN,DPD levels in ovariectomized rats.Conclusions The potential protective effect of FLL is due to an increased bone formation with reduction in bone resorption in OVX rats.

关 键 词：女贞子 去卵巢 骨质疏松 骨转换 骨密度 

分 类 号：R-332[医药卫生] R282.71