慢性吗啡处理对大鼠交感神经节pCREB和CREB mRNA表达的影响  

Effects of chronic morphine treatment on expression of pCREB and CREB mRNA in sympathetic ganglia of rats

作　　者：何萍[1] 陈家欢[2] 黄晓亮[2] 于洪波[1] 莫宁[1] 

机构地区：[1]广西医科大学药学院药理学教研室,广西南宁530021 [2]广西医科大学药学院中心实验室,广西南宁530021

出　　处：《中国药理学通报》2013年第2期238-241,共4页Chinese Pharmacological Bulletin

基　　金：广西自然科学基金青年基金资助项目(No桂科青0542045);广西医科大学博士启动基金资助项目(No308054)

摘　　要：目的观察慢性吗啡(Mor)处理对大鼠交感神经节-颈上神经节(SCG)环腺苷酸反应元件结合蛋白(cAMP responseelement binding protein,CREB)磷酸化和mRNA表达的影响。方法 Wistar大鼠随机分成4组(正常对照组、吗啡急性给药组、吗啡依赖组、吗啡戒断组),免疫组织化学方法及RT-PCR法分别检测磷酸化的CREB(phosphorylated CREB,pCREB)和CREB mRNA在SCG中的表达。结果 (1)与正常对照组相比,吗啡急性给药组大鼠SCG中pCREB含量明显降低(P<0.05);(2)吗啡依赖组大鼠SCG中pCREB含量回到正常对照组水平,并有增高趋势(与正常对照组比较,P>0.05;与吗啡急性给药组比较,P<0.01);(3)吗啡戒断组大鼠SCG中pCREB含量明显高于正常对照组(P<0.01);(4)各组大鼠SCG的CREBmRNA表达无差异(P>0.05)。结论慢性吗啡处理大鼠交感神经节CREB磷酸化水平存在适应性上调现象。Aim To study the effects of chronic morphine treatment on the expression of phosphorylated cAMP response element binding protein（pCREB） and CREB mRNA in sympathetic ganglia,superior cervical ganglia（SCG） of rats.Methods The Wistar rats were randomly divided into 4 groups,namely control, morphine acute,morphine dependent and morphine withdrawal group.The expression of pCREB and CREB mRNA in SCG was detected by means of immunohistochemistry and RT-PCR respectively.Results（1） The content of pCREB in SCG of morphine-acute group was lower than that of control group（P0.05）.（2） The content of pCREB in SCG of morphine dependent group returned to the level of control group with an increasing tendency（compared with control,P0.05;compared with morphine-acute,P0.01）.（3） Compared with control group,the content of pCREB in SCG of morphine withdrawal group was increased（P0.01）.（4） The expressive level of CREB mRNA in SCG was not significantly changed in each group（P0.05）.Conclusion There is an up-regulation of pCREB in sympathetic ganglia of chronic morphine treated rats.

关 键 词：吗啡 依赖 耐受 戒断 交感神经节 PCREB CREB mRNA 

分 类 号：R-332[医药卫生] R322.85