莪术醇对人卵巢癌SKOV3细胞株JAK2/STAT3信号通路影响的研究  

Research on the effect of Curcumol in JAK2/STAT3 signaling pathway in human ovarian cancer cell line SKOV3

作　　者：汤欣[1] 韩凤娟[1] 李威[1] 王龙[1] 蔡冬燕[1] 吴效科[1] 

机构地区：[1]黑龙江中医药大学附属第一医院,哈尔滨150040

出　　处：《中国妇产科临床杂志》2013年第1期43-46,共4页Chinese Journal of Clinical Obstetrics and Gynecology

基　　金：教育部“春晖计划”(Z2007-1-15001);黑龙江自然科学基金面上项目(D200816);国家自然科学基金面上项目(81273788)

摘　　要：目的观察莪术醇对人卵巢癌SKOV3细胞株体外生长的影响及对SKOV3细胞株JAK2/STAT3信号通路基因表达的影响,探讨莪术醇治疗卵巢癌的分子机制。方法不同浓度莪术醇作用于人卵巢癌细胞株SKOV3,MTT法检测SKOV3细胞的增殖抑制率,实时荧光定量-PCR法检测SKOV3细胞中JAK2、STAT3基因表达水平。结果莪术醇对卵巢癌SKOV3细胞有明显的增殖抑制作用,其强度随药物作用时间及浓度的增加而增强,呈时间-剂量依赖性。实时荧光定量-PCR法检测证实莪术醇作用后SKOV3细胞中JAK2、STAT3基因表达受到明显抑制。结论莪术醇能明显抑制SKOV3细胞的体外增殖,诱导细胞凋亡,其机制可能是通过降调节JAK2、STAT3基因的表达来实现的。Objective To observe the effect of Curcumol in human ovarian cancer cell line SKOV3 in vitro and the molecular mechanism. Methods The proliferation inhibition rate of human ovarian cancer SKOV3 cell was detected by MTT and JAK2/STAT3 gene expression by real-time fluorescent quantitative-PCR after treated with different concentrations of Curcumol.Results Curcumol significantly inhibited the proliferation of ovarian cancer SKOV3 cell. The intensity increased with the time and concentration of drug increased with a time-dose dependent. JAK2/STAT3 gene expression was significantly inhibited in SKOV3 cell after treated with Curcumol.Conclusions Curcumol can inhibit SKOV3 cell proliferation in vitro and induce apoptosis. The mechanism may be related to the down-regulation of JAK2/STAT3 gene.

关 键 词：莪术醇 人卵巢癌细胞株SKOV3 增殖抑制 JAK2 STAT3信号通路 实时荧光定量-PCR 

分 类 号：R737.31[医药卫生—肿瘤]