Th17细胞及其相关因子在活动期溃疡性结肠炎患者中的表达及意义  

Significance of number of Th17 cells and expression of Th17-related cytokines in patients with active ulcerative colitis

作　　者：张晓斐[1] 蒋文瑜[1] 于鹏丽[1] 李文杰[1] 张红杰[1] 

机构地区：[1]南京医科大学第一附属医院消化科,江苏省南京市210029

出　　处：《世界华人消化杂志》2013年第1期19-26,共8页World Chinese Journal of Digestology

基　　金：兴卫工程重点人才基金资助项目;No.RC201163~~

摘　　要：目的:检测Th17细胞及其相关因子白介素17(interleukin-17,IL-17)、IL-21在活动期溃疡性结肠炎(ulcerative colitis,UC)患者中的分布与表达,并分析其与疾病活动度、C-反应蛋白(C-reactive protein,CRP)、血沉(erythrocyte sedimentation rate,ESR)、内镜下活动度分级以及组织病理分级的关系.方法:免疫荧光双标法检测40例活动期UC患者及20例健康对照者肠黏膜组织中Th17细胞的分布,免疫组织化学SP法检测上述患者肠黏膜组织中IL-17、IL-21的表达与分布,酶联免疫吸附法检测血清IL-17、IL-21水平.结果:活动期UC患者肠黏膜固有层中Th17细胞数明显增多.活动期UC患者肠黏膜组织及血清中IL-17、IL-21的表达均明显高于对照组(分别为:0.0087±0.0019vs0.0008±0.0001,P<0.05;0.0082±0.0017vs0.0005±0.0001,P<0.01),且IL-17、IL-21的表达在轻、中、重度患者间均有显著性差异(分别为:0.0020±0.0004vs0.0079±0.0016vs0.0173±0.0011;0.0020±0.0004vs0.0097±0.0002vs0.0120±0.0006),随疾病活动度增加而逐渐增强.IL-17、IL-21的表达与活动期UC患者CRP水平、内镜下活动度分级、组织病理分级均显著正相关(P<0.05),IL-17的表达与活动期UC患者的ESR水平显著正相关(P<0.05).结论:Th17细胞及其相关因子IL-17、IL-21在活动期UC患者高表达,且随疾病活动程度增加而逐渐增强.Th17细胞及其相关因子与UC的疾病活动与黏膜损伤有关.AIM：To investigate the association of number of Th17 cells and expression of Th17-related cytokines（IL-17 and IL-21） with disease activity,C-reactive protein（CRP）,erythrocyte sedimentation rate（ESR）,endoscopic stage,and histological grade in patients with active ulcerative colitis（UC）.METHODS：Forty patients with active UC and 20 healthy controls were recruited.The infiltration of Th17 cells in colonic mucosa was assessed by immunofluorescence.The expression of IL-17 and IL-21 was detected by immunohistochemistry.Serum levels of IL-17 and L-21 were determined by ELISA.RESULTS：Compared to healthy controls,the number of Th17 cells increased mainly in the lamina propria of active UC patients.The expression of IL-17 and IL-21 in the colonic mucosa and serum levels of these two cytokines in active UC patients were significantly higher than those in normal controls（0.0087±0.0019 vs 0.0008±0.0001,P0.05;0.0082±0.0017 vs 0.0005±0.0001,P0.01）.The expression of IL-17 and IL-21 was higher in severe UC than in moderate or mild UC（0.0020±0.0004 vs 0.0079±0.0016 vs 0.0173±0.0011;0.0020±0.0004 vs 0.0097±0.0002 vs 0.0120±0.0006）.IL-17 and IL-21 expression was positively correlated with CRP,endoscopic stage,and histological grade in patients with active UC（all P0.05）.The expression of IL-17 was also positively correlated with ESR（P0.05）.CONCLUSION：The number of Th17 cells and expression of Th17-related cytokines（IL-17 and IL-21） increase in the colonic mucosa of patients with active UC.Th17 cells and Th17-related cytokines may play an important role in disease activity and mucosal damage in patients with active UC.

关 键 词：溃疡性结肠炎 TH17 白介素17 白介素21 

分 类 号：R574.62[医药卫生—消化系统]