大鼠视神经部分切断模型的建立和重复性评价  

The establishment of a rat partial optic nerve transection model and assessment of its reproducibility

作　　者：颜繁诚[1] 李树宁[1] 刘克高[1] 卢清君[1] 王宁利[1] 

机构地区：[1]首都医科大学附属北京同仁医院北京同仁眼科中心北京市眼科学与视觉科学重点实验室,100730

出　　处：《眼科》2013年第1期34-37,共4页Ophthalmology in China

基　　金：国家自然科学基金青年科学基金项目(30700921)

摘　　要：目的应用自行设计的模具制作大鼠视神经部分切断模型,并评价大鼠视神经部分切断模型的可重复性。设计实验研究。研究对象15只Wistar大鼠。方法利用模具将大鼠视神经部分切断,术后对13只大鼠行荧光金逆行标记及全视网膜拼图,使用Ret-camⅡ眼底照相观察视网膜血供情况。主要指标观察视网膜神经节细胞(RGC)形态及分布变异。结果视神经部分切断直接影响的视网膜与周围正常视网膜有明确分界线,标记荧光金视网膜面积比例变异系数最大值为1.85%,平均变异系数为0.67%±0.44%。Ret-camⅡ眼底照相显示视神经部分切断后,未引起视网膜供血障碍。结论利用新型模具器械可成功建立易于量化的重复性高的RGC继发性损伤模型。Objective To design an instrument to complish helpfully partial optic nerve transection model and evaluate the repeatability of the transection model in rat. Design Experimental study. Participants 15 healthy Wistar rats. Methods The rat partial optic nerve transection model was established with the new designed rat optic instrument. After 13 models established successfully, fluorogold（FG） was used to label retinal ganglion cells of each model. The images of retinal ganglion cells in the retina were captured with fluorescence microscope. The retina blood circulation of the model was recorded with Ret-cam. Main Outcome Measures The morphology and distribution of retinal ganglion cells in the retina of each model was observed and measured. Results The measurement of unlabled FG retina area showed that the boundaries of unlabled FG retina were clear. The maximum coefficient of variation was 1.85%, average coefficient of variation 0.67%±0.44 %. Additionally, in the process of partial optic nerve transection, there was no retinal ischemia phenomenon. Conclusion This new rat optic instrument can be easily used to establish the partial optic nerve transection model with a satisfactory reproducibility. （Ophthalmol CHN, 2013, 22： 34-37）

关 键 词：视神经部分切断模型 大鼠 视网膜神经节细胞 继发性损伤 

分 类 号：R779.1[医药卫生—眼科]