ALDH1A2、MMP2和E-cadherin在结肠癌组织中的表达及相关性  

Significance of ALDH1A2,MMP2 and E-cadherin expression in colonic cancer

作　　者：杨万广[1] 王万鹏[1] 张谢夫[2] 

机构地区：[1]郑州大学第一附属医院急诊外科,河南省郑州市450052 [2]郑州大学第一附属医院胃肠外科,河南省郑州市450052

出　　处：《世界华人消化杂志》2013年第4期362-366,共5页World Chinese Journal of Digestology

摘　　要：目的:研究结肠癌组织中ALDH1A2、E-cadherin和MMP2的表达及与临床病理特征之间的关系.方法:应用免疫组织化学SP法、逆转录聚合酶链反应(RT-PCR)、免疫印迹(Western blot)检测52例结肠癌及癌旁组织中ALDH1A2、E-cadherin和MMP2蛋白的表达,分析其间的相关性以及与肿瘤临床病理特征之间的关系.结果:免疫组织化学测定显示ALDH1A2在52例结肠癌癌旁组织阳性率为67.31%;E-cadherin在52例结肠癌癌旁阳性率为90.38%;癌旁组织ALDH1A2、E-cadherin的表达率高于癌组织.MMP2在52例结肠癌组织中阳性率占86.54%,高于癌旁组织(P<0.05).逆转录-PCR及免疫印迹技术显示同样结果.ALDH1A2、MMP2及E-cadherin在结肠癌中的表达具有相关性.结论:结肠癌中ALDH1A2、E-cadherin表达低于癌旁组织,MMP2表达高于癌旁组织,ALDH1A2、MMP2及E-cadherin三者密切相关.AIM：To investigate the expression of ALDH1A2,E-cadherin and MMP2 in colonic cancer and to analyze their clinical significance.METHODS：RT-PCR,Western blot and immunohistochemistry were used to examine the expression and distribution of ALDH1A2,E-cadherin and MMP2 in 52 surgically resected specimens of colonic cancer and matched tumor-adjacent colonic mucosal tissue.The correlations among them and their relationship to the clinicopathologic characteristics of colonic cancer were analyzed.RESULTS：The positive rates of ALDH1A2 and E-cadherin in tumor-adjacent colonic mucosal tissue were 67.31% and 90.38%,respectively,which were significantly higher than that in colonic cancer tissue.The positive rate of MMP2 in colonic cancer was 86.54%,significantly higher than that in tumor-adjacent colonic mucosal（P0.05）.RT-PCR and Western blot analyses revealed similar results.There were significant correlations among ALDH1A2,MMP2 and E-cadherin expression in colorectal carcinoma.CONCLUSION：Expression of ALDH1A2 and E-cadherin in colonic cancer is lower than in tumor-adjacent colonic mucosal tissue,while MMP2 expression in colonic cancer is higher than that in tumor-adjacent colonic mucosal tissue.ALDH1A2 expression was correlated with MMP2 and E-cadherin expression in colonic cancer.

关 键 词：ALDH1A2 金属基质蛋白酶2 上皮钙黏蛋白 结肠癌 

分 类 号：R735.35[医药卫生—肿瘤]