Synthesis of NAD analogs to develop bioorthogonal redox system  

出　　处：《Science China(Physics,Mechanics & Astronomy)》2013年第3期296-300,共5页中国科学：物理学、力学、天文学（英文版）

基　　金：supported by the National Basic Research Program of China (2012CB721103);;the National Natural Science Foundation of China (21102143)

摘　　要：Three new nicotinamide adenine dinucleotide(NAD) analogs were synthesized,and their characteristics as cofactors for Escherichia coli malic enzyme(ME) and its double mutant ME L310R/Q401C were analyzed.Each pair of the NAD analog and the double mutant showed good orthogonality to the natural pair of NAD and ME in terms of catalyzing oxidative decarboxylation of L-malic acid.Results indicated that molecular interactions between redox enzyme and cofactor could be further explored to generate new bioorthogonal redox systems.Three new nicotinamide adenine dinucleotide （NAD） analogs were synthesized, and their characteristics as cofactors for Esch- erichia coli malic enzyme （ME） and its double mutant ME L310R/Q401C were analyzed. Each pair of the NAD analog and the double mutant showed good orthogonality to the natural pair of NAD and ME in terms of catalyzing oxidative decarboxylation of L-malic acid. Results indicated that molecular interactions between redox enzyme and cofactor could be further explored to generate new bioorthogonal redox systems.

关 键 词：NAD analog bioorthogonal OXIDOREDUCTASES chemical biology 

分 类 号：Q942.5[生物学—植物学] TS941.26[轻工技术与工程—服装设计与工程]