高浓度医用臭氧对大鼠运动功能和脊髓Bax、Bcl-2表达的影响  

EFFECTS OF INTRATHECAL ADMINISTRATION OF HIGH CONCENTRATION OF OZONE ON THE RATS BEHAVIOR AND EXPRESSION OF Bax,Bcl-2 IN SPINAL CORD

作　　者：许维澄[1] 林小雯[2] 王群波[1] 傅志俭[2] 

机构地区：[1]重庆医科大学附属永川医院骨科,重庆402160 [2]山东省立医院疼痛科,济南250021

出　　处：《中国疼痛医学杂志》2013年第2期95-97,共3页Chinese Journal of Pain Medicine

基　　金：国家自然科学基金资助项目(30972844、81271346);山东省自然科学基金资助项目(Y2006C69)

摘　　要：目的:观察鞘内注射高浓度医用臭氧对大鼠运动功能和脊髓组织中Bax、Bcl-2基因表达的影响。方法:SD大鼠16只,随机均分为2组(n=8),分别建立大鼠鞘内置管模型。对照组(C组)仅行鞘内穿刺置管不注射;医用臭氧组(O3-60组):鞘内置管后经导管注射60μg/mL医用臭氧10μL。麻醉前及注射医用臭氧后24 h分别对两组大鼠进行BBB运动功能评分;然后立即断颈取材,采用Western blot测定腰膨大处脊髓组织Bax、Bcl-2蛋白表达水平。结果:与对照组相比,鞘内注射高浓度医用臭氧24 h后,臭氧组大鼠后肢BBB运动功能评分(Basso Beattie andBresnahan locomotor rating scales,BBB scores)显著降低(P<0.05);脊髓Bax蛋白表达显著增加(P<0.05),Bcl-2/Bax比值显著降低(P<0.05)。结论:高浓度医用臭氧鞘内注射可损伤大鼠运动功能,其损伤可能与神经细胞凋亡有关。Objective： To investigate the effects of intrathecal administration of high concentration of ozone on the animal behavior and protein expression of Bax and Bcl-2 in spinal cord of rats. Methods： Sixteen healthy adult Sprague-Dawley rats were randomly divided into two groups （n = 8）： the control group （Group C） and the 60μg/mL 03 group （Group O3-60i. In Group C, rats were intrathecally catheterized without any medication, while in the Group 03-60, 60 μg/ml oxygen-ozone 10 μL was administered intrathecally. The BBB locomotor rating scales （the BBB score） were recorded before anesthesia and 24h after 03-60 administered. The lumbar spinal cord was collected to detect the protein expression of Bax and Bcl-2 by western blot. Results： After intrathecal 03-60 administration, compared with Group C, the BBB score of Group 03-60 was significantly lower （P 〈 0.05）. The expression of Bax was significantly higher （P 〈 0.05）, and the ratio of Bax/Bcl-2 of Group 03-60 was significantly lower （P 〈 0.05）. Conclusion： The intrathecal administration of high concentration of ozone might damage the behavior of rats, and the neurotoxicity of ozone may be related to the procession of neuron apoptosis.

关 键 词：医用臭氧 神经细胞凋亡 BAX BCL-2 

分 类 号：R363[医药卫生—病理学]