蛛网膜下腔出血后大鼠脑内白细胞介素-1β水平及其与继发性脑水肿的关系  

The contain of IL -1β in rat brain after subarachnoid hemorrhage and its relation to secondary brain edema

作　　者：冯力[1] 李中林[1] 纪培志[1] 颜丙超[1] 

机构地区：[1]徐州医学院附属医院神经外科,江苏省徐州221002

出　　处：《中国基层医药》2013年第2期197-199,共3页Chinese Journal of Primary Medicine and Pharmacy

摘　　要：目的探讨蛛网膜下腔出血（SAH）后白细胞介素-1β（IL-1β）的表达变化以及与脑水肿、脑血管痉挛的关系。方法通过大脑前动脉穿刺法制作大鼠SAH模型，用于重-湿重法测定蛛网膜下腔出血后脑水肿的变化情况，HE染色观察基底动脉的血管形态学变化，Westernblot实验检测大鼠脑组织IL-1β的表达情况。结果SAH后大鼠脑组织水肿从1d开始，7d时最明显，以后逐渐减轻。IL-1β蛋白水平逐渐增高，术后7d达高峰，之后逐渐降低，14d时仍维持较高水平。IL-1β水平和脑水肿的变化具有相关性，而与血管痉挛的程度没有相关性。结论大鼠蛛网膜下腔出血后IL-1β水平增高，7d达到高峰；脑组织IL-1β表达变化与脑水肿程度呈正相关，表明IL一1B可能参与蛛网膜下腔出血后脑水肿的形成。Objective To investigate the changes of interleukin-1β （IL-1β） in rat brain after subarachnoid hemorhage（SAH） and its relation to cerebral edema and vasospasm. Methods Subarachnoid hemorrhage rat model was produced by anterior cerebral artery perforation method. The brain water content was measured by dry-wet weight method. Hematoxylin-eosin（HE） staining was used to assess vasospasm in the basilar artery,and the expression of IL- l13 after SAI-I was examined by Western blot. Results After SAH, the cerebral edema gradually occured from 1 d, reached the peak at 7d. The level of IL-1β protein started to increase in 1 d, reached its peak in 7d, and remained at a relative high level in 14d. There＇s correlation between the level of IL-1β protein and changes of cerebral edema. Conclusion After SAH, The level of IL-1β gradually rised and fits remarkably well with the time course of cerebral edema,which may indicate that IL-1β leads to the development of cerebral edema after SAH.

关 键 词：白细胞介素-1Β 蛛网膜下腔出血 脑水肿 模型 动物 

分 类 号：R74[医药卫生—神经病学与精神病学]